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PMID: 2477686 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Induction of the TRPM-2 gene in cells undergoing programmed death.

Molecular and cellular biology ·Vol. 9 ·No. 8 ·1989-08-00 ·Pages 3473-81

Buttyan R, Olsson CA, Pintar J, Chang C, Bandyk M, Ng PY, Sawczuk IS

Abstract

RNA and protein products encoded by the testosterone-repressed prostate message-2 gene (TRPM-2) are induced to high levels, coordinate with the onset of cell death, in numerous rodent models of inducible tissue damage. These models include cell death initiated by hormonal stimuli (prostate regression), pressure insult (renal atrophy after ureteral obstruction), developmental stimuli (necrosis of interdigital tissue), and cytotoxic injury (chemotherapeutic regression of a tumor). Sequence analysis of cDNA encoding TRPM-2 revealed its close homology with a product referred to as SGP-2 or clusterin expressed constitutively by Sertoli cells; however, the immunologically related polypeptides expressed in regressing tissues differ in molecular mass from the forms secreted by the testis. Although the function(s) of the products encoded by the TRPM-2 gene remains unclear, their presence provides a remarkable and early indicator of programmed cell death in many types of mammalian cells.

MeSH Terms
Animals Autoradiography Cell Survival Clusterin Extremities/embryology Female Gene Expression Regulation Glycoproteins/genetics Kidney/cytology Male Mice Mice, Inbred C3H Molecular Chaperones Nucleic Acid Hybridization Prostate/cytology RNA RNA, Antisense Rats Rats, Inbred Strains Sequence Homology, Nucleic Acid Sertoli Cells/immunology,metabolism Testosterone/physiology Ureteral Obstruction/pathology Urinary Bladder Neoplasms/genetics
Chemicals
Clu protein, mouse Clusterin Glycoproteins Molecular Chaperones RNA, Antisense Testosterone RNA
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Buttyan R
Department of Urology, Columbia University College of Physicians and Surgeons, New York, New York 10032.
Olsson C A
Pintar J
Chang C
Bandyk M
Ng P Y
Sawczuk I S
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.9.8.3473-3481.1989
Published
1989-08-00
Pages
3473-81
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC362394
Subset
IM
Grants
NCI NIH HHS · CA47848 · United States
NIDDK NIH HHS · DK40832 · United States
NINDS NIH HHS · NS21970 · United States
External Links
PubMed source DOI: 10.1128/mcb.9.8.3473-3481.1989 PMC Full Text View journal details
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