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PMID: 23912276 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Dimeric WH2 domains in Vibrio VopF promote actin filament barbed-end uncapping and assisted elongation.

Nature structural & molecular biology ·Vol. 20 ·No. 9 ·2013-09-00 ·Pages 1069-76

Pernier J, Orban J, Avvaru BS, Jégou A, Romet-Lemonne G, Guichard B, Carlier MF

Abstract

Proteins containing repeats of the WASP homology 2 (WH2) actin-binding module are multifunctional regulators of actin nucleation and assembly. The bacterial effector VopF in Vibrio cholerae, like VopL in Vibrio parahaemolyticus, is a unique homodimer of three WH2 motifs linked by a C-terminal dimerization domain. We show that only the first and third WH2 domains of VopF bind G-actin in a non-nucleating, sequestered conformation. Moreover, dimeric WH2 domains in VopF give rise to unprecedented regulation of actin assembly. Specifically, two WH2 domains on opposite protomers of VopF direct filament assembly from actin or profilin-actin by binding terminal subunits and uncapping capping protein from barbed ends by a new mechanism. Thus, VopF does not nucleate filaments by capping a pointed-end F-actin hexamer. These properties may contribute to VopF pathogenicity, and they show how dimeric WH2 peptides may mediate processive filament growth.

MeSH Terms
Actin Capping Proteins/chemistry,metabolism Actins/chemistry,metabolism Animals Bacterial Proteins/chemistry,genetics,metabolism Models, Molecular Protein Conformation Protein Interaction Domains and Motifs Protein Multimerization Protein Structure, Quaternary Rabbits Recombinant Proteins/chemistry,genetics,metabolism Vibrio cholerae/genetics,metabolism Wiskott-Aldrich Syndrome Protein Family/chemistry,genetics,metabolism
Chemicals
Actin Capping Proteins Actins Bacterial Proteins Recombinant Proteins Wiskott-Aldrich Syndrome Protein Family
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Pernier Julien
Cytoskeleton Dynamics and Motility Group, Laboratoire d'Enzymologie et Biochimie Structurales, Centre National de la Recherche Scientifique, Gif-sur-Yvette, France.
Orban Jozsef
Avvaru Balendu Sankara
Jégou Antoine
Romet-Lemonne Guillaume
Guichard Bérengère
Carlier Marie-France
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Article Info
Journal
Nature structural & molecular biology
Abbr.
Nat Struct Mol Biol
ISSN
1545-9985
DOI
10.1038/nsmb.2639
Published
2013-09-00
Epub
2013-00-04
Pages
1069-76
Language
English
Region
United States
NLM ID
101186374
Subset
IM
External Links
PubMed source DOI: 10.1038/nsmb.2639 View journal details
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