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PMID: 2391360 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Fimbrin is a homologue of the cytoplasmic phosphoprotein plastin and has domains homologous with calmodulin and actin gelation proteins.

The Journal of cell biology ·Vol. 111 ·No. 3 ·1990-09-00 ·Pages 1069-79

de Arruda MV, Watson S, Lin CS, Leavitt J, Matsudaira P

Abstract

Fimbrin is an actin-bundling protein found in intestinal microvilli, hair cell stereocilia, and fibroblast filopodia. The complete protein sequence (630 residues) of chicken intestine fimbrin has been determined from two full-length cDNA clones. The sequence encodes a small amino-terminal domain (115 residues) that is homologous with two calcium-binding sites of calmodulin and a large carboxy-terminal domain (500 residues) consisting of a fourfold-repeated 125-residue sequence. This repeat is homologous with the actin-binding domain of alpha-actinin and the amino-terminal domains of dystrophin, actin-gelation protein, and beta-spectrin. The presence of this duplicated domain in fimbrin links actin bundling proteins and gelation proteins into a common family of actin cross-linking proteins. Fimbrin is also homologous in sequence with human L-plastin and T-plastin. L-plastin is found in only normal or transformed leukocytes where it becomes phosphorylated in response to IL 1 or phorbol myristate acetate. T-plastin is found in cells of solid tissues where it does not become phosphorylated. Neoplastic cells derived from solid tissues express both isoforms. The differences in expression, sequence, and phosphorylation suggest possible functional differences between fimbrin isoforms.

MeSH Terms
Actins/metabolism Amino Acid Sequence Animals Base Sequence Calcium/metabolism,physiology Calcium-Binding Proteins/genetics Calmodulin/genetics Carrier Proteins/genetics Chickens Cross Reactions Gene Expression Regulation Membrane Glycoproteins/genetics Microfilament Proteins/genetics Molecular Sequence Data Phosphoproteins/genetics Phosphorylation Repetitive Sequences, Nucleic Acid Sequence Homology, Nucleic Acid Structure-Activity Relationship
Chemicals
Actins Calcium-Binding Proteins Calmodulin Carrier Proteins F-actin-binding proteins Membrane Glycoproteins Microfilament Proteins Phosphoproteins plastin villin Calcium
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
de Arruda M V
Whitehead Institute for Biomedical Research, Cambridge, Massachusetts.
Watson S
Lin C S
Leavitt J
Matsudaira P
References (50)
50 references, click to expand
  1. Isolation and properties of actin, myosin, and a new actinbinding protein in rabbit alveolar macrophages.
    J Biol Chem. 1975 Jul 25;250(14):5696-705 PMID: 124734
  2. The structure and function of alpha-actinin.
    J Muscle Res Cell Motil. 1989 Aug;10(4):280-9 PMID: 2671039
  3. The gelation of actin by actin-binding protein.
    J Biol Chem. 1978 Dec 25;253(24):8988-93 PMID: 721823
  4. Separation and interaction of the major components of sea urchin actin gel.
    J Mol Biol. 1978 Oct 25;125(2):207-24 PMID: 731692
  5. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.
    Biochemistry. 1979 Nov 27;18(24):5294-9 PMID: 518835
  6. Identification and organization of the components in the isolated microvillus cytoskeleton.
    J Cell Biol. 1979 Dec;83(3):667-73 PMID: 574874
  7. Villin is a major protein of the microvillus cytoskeleton which binds both G and F actin in a calcium-dependent manner.
    Cell. 1980 Jul;20(3):839-47 PMID: 6893424
  8. Fimbrin, a new microfilament-associated protein present in microvilli and other cell surface structures.
    J Cell Biol. 1980 Jul;86(1):335-40 PMID: 6998986
  9. Demonstration of at least two different actin-binding sites in villin, a calcium-regulated modulator of F-actin organization.
    J Biol Chem. 1981 Aug 10;256(15):8156-61 PMID: 6790532
  10. F-actin binding and bundling properties of fimbrin, a major cytoskeletal protein of microvillus core filaments.
    J Biol Chem. 1981 Sep 10;256(17):9283-8 PMID: 6894925
  11. Calmodulin localization during capping and receptor-mediated endocytosis.
    Nature. 1981 Nov 12;294(5837):163-6 PMID: 7029305
  12. Fimbrin is a cytoskeletal protein that crosslinks F-actin in vitro.
    Proc Natl Acad Sci U S A. 1981 Nov;78(11):6849-53 PMID: 6947259
  13. Demonstration of three distinct calcium-binding sites in villin, a modulator of actin assembly.
    J Biol Chem. 1983 Jan 10;258(1):365-9 PMID: 6848508
  14. Tissue-specific expression of a chicken calmodulin pseudogene lacking intervening sequences.
    Proc Natl Acad Sci U S A. 1983 Nov;80(21):6485-9 PMID: 6579534
  15. A comprehensive set of sequence analysis programs for the VAX.
    Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):387-95 PMID: 6546423
  16. Properties of the 120,000- and 95,000-dalton actin-binding proteins from Dictyostelium discoideum and their possible functions in assembling the cytoplasmic matrix.
    J Cell Biol. 1984 Jul;99(1 Pt 2):119s-126s PMID: 6746725
  17. The structural organization of the chicken calmodulin gene.
    J Biol Chem. 1985 Jan 25;260(2):907-12 PMID: 2981850
  18. Partial purification and characterization of an actin-bundling protein, band 4.9, from human erythrocytes.
    J Cell Biol. 1985 Mar;100(3):775-85 PMID: 3882722
  19. Bacterial expression and characterization of proteins derived from the chicken calmodulin cDNA and a calmodulin processed gene.
    J Biol Chem. 1985 Apr 25;260(8):4704-12 PMID: 2985564
  20. Purification and characterization of an F-actin-bundling 55-kilodalton protein from HeLa cells.
    J Biol Chem. 1985 Apr 25;260(8):5087-97 PMID: 3886649
  21. Specific protein phosphorylation in human promyelocytic HL-60 leukemia cells susceptible or resistant to induction of cell differentiation by phorbol-12-myristate-13-acetate.
    Cancer Res. 1985 Oct;45(10):4955-62 PMID: 3861240
  22. Mapping the cysteine residues and actin-binding regions of villin by using antisera to the amino and carboxyl termini of the molecule.
    Proc Natl Acad Sci U S A. 1985 Oct;82(20):6788-92 PMID: 2413446
  23. Abundant synthesis of the transformation-induced protein of neoplastic human fibroblasts, plastin, in normal lymphocytes.
    Cancer Res. 1985 Nov;45(11 Pt 2):5643-7 PMID: 4053036
  24. F-actin aggregates in transformed cells contain alpha-actinin and fimbrin but apparently lack tropomyosin.
    Eur J Cell Biol. 1986 Jan;39(2):313-20 PMID: 3007147
  25. Isolation and characterization of a conserved actin-binding domain from rat hepatic actinogelin, rat skeletal muscle, and chicken gizzard alpha-actinins.
    J Biol Chem. 1986 Aug 15;261(23):10680-7 PMID: 3733725
  26. Actin and actin-binding proteins. A critical evaluation of mechanisms and functions.
    Annu Rev Biochem. 1986;55:987-1035 PMID: 3527055
  27. Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes.
    J Mol Biol. 1986 May 5;189(1):113-30 PMID: 3537305
  28. Improved method for mapping the binding site of an actin-binding protein in the actin sequence. Use of a site-directed antibody against the N-terminal region of actin as a probe of its N-terminus.
    Biochemistry. 1986 Oct 7;25(20):6186-92 PMID: 3790514
  29. Synapsin I bundles F-actin in a phosphorylation-dependent manner.
    Nature. 1987 Apr 16-22;326(6114):704-7 PMID: 3104800
  30. Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes.
    J Biol Chem. 1987 Jul 25;262(21):10035-8 PMID: 3611052
  31. Crystal structures of the helix-loop-helix calcium-binding proteins.
    Annu Rev Biochem. 1989;58:951-98 PMID: 2673026
  32. Sequence similarity of the amino-terminal domain of Drosophila beta spectrin to alpha actinin and dystrophin.
    J Cell Biol. 1989 Oct;109(4 Pt 1):1633-41 PMID: 2677025
  33. The spectrin membrane skeleton: emerging concepts.
    Curr Opin Cell Biol. 1989 Feb;1(1):23-9 PMID: 2698206
  34. The function of the major cytoskeletal components of the brush border.
    Curr Opin Cell Biol. 1989 Feb;1(1):51-7 PMID: 2698209
  35. Correction of the N-terminal sequences of the human plastin isoforms by using anchored polymerase chain reaction: identification of a potential calcium-binding domain.
    Mol Cell Biol. 1990 Apr;10(4):1818-21 PMID: 2378651
  36. Calcium-sensitive non-muscle alpha-actinin contains EF-hand structures and highly conserved regions.
    FEBS Lett. 1987 Sep 14;221(2):391-6 PMID: 3622778
  37. Protein sequence of DMD gene is related to actin-binding domain of alpha-actinin.
    Cell. 1987 Oct 9;51(1):1 PMID: 3652206
  38. The sequence of chick alpha-actinin reveals homologies to spectrin and calmodulin.
    J Biol Chem. 1987 Dec 25;262(36):17623-9 PMID: 2826427
  39. alpha-Actinins and the DMD protein contain spectrin-like repeats.
    Cell. 1988 Jan 29;52(2):159-60 PMID: 3342446
  40. The complete sequence of dystrophin predicts a rod-shaped cytoskeletal protein.
    Cell. 1988 Apr 22;53(2):219-28 PMID: 3282674
  41. Substructure and higher structure of chicken smooth muscle alpha-actinin molecule.
    J Biol Chem. 1988 Jun 5;263(16):7800-5 PMID: 3286641
  42. Pieces in the actin-severing protein puzzle.
    Cell. 1988 Jul 15;54(2):139-40 PMID: 2839297
  43. Villin sequence and peptide map identify six homologous domains.
    Proc Natl Acad Sci U S A. 1988 Jul;85(14):4986-90 PMID: 2839826
  44. Purification and characterization of a cytosolic 65-kilodalton phosphoprotein in human leukocytes whose phosphorylation is augmented by stimulation with interleukin 1.
    Biochemistry. 1988 May 17;27(10):3765-70 PMID: 3261603
  45. Abolition of actin-bundling by phosphorylation of human erythrocyte protein 4.9.
    Nature. 1988 Aug 25;334(6184):718-21 PMID: 2842686
  46. Molecular cloning and characterization of plastin, a human leukocyte protein expressed in transformed human fibroblasts.
    Mol Cell Biol. 1988 Nov;8(11):4659-68 PMID: 3211125
  47. The chicken dystrophin cDNA: striking conservation of the C-terminal coding and 3' untranslated regions between man and chicken.
    EMBO J. 1988 Dec 20;7(13):4157-62 PMID: 3072195
  48. The Dictyostelium gelation factor shares a putative actin binding site with alpha-actinins and dystrophin and also has a rod domain containing six 100-residue motifs that appear to have a cross-beta conformation.
    J Cell Biol. 1989 Aug;109(2):607-18 PMID: 2668299
  49. Association of type 3 protein kinase C with focal contacts in rat embryo fibroblasts.
    J Cell Biol. 1989 Aug;109(2):697-704 PMID: 2668303
  50. Filamin, a new high-molecular-weight protein found in smooth muscle and non-muscle cells.
    Proc Natl Acad Sci U S A. 1975 Nov;72(11):4483-6 PMID: 53835
Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.111.3.1069
Published
1990-09-00
Pages
1069-79
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2116281
Subset
IM
Grants
NCI NIH HHS · CA-34763 · United States
NCI NIH HHS · CA-44704 · United States
NIDDK NIH HHS · DK-35306 · United States
Databases
GENBANK
X52562, X5262
External Links
PubMed source DOI: 10.1083/jcb.111.3.1069 PMC Full Text View journal details
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