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PMID: 6579534 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Tissue-specific expression of a chicken calmodulin pseudogene lacking intervening sequences.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 80 ·No. 21 ·1983-11-00 ·Pages 6485-9

Stein JP, Munjaal RP, Lagace L, Lai EC, O'Malley BW, Means AR

Abstract

An eel calmodulin cDNA probe has been used to isolate a calmodulin gene from a chicken DNA library. Sequence analysis revealed this calmodulin gene (cCM1) to contain the nucleotides that code for 148 amino acids, a termination codon, and 486 residues of 3'-noncoding sequence before an A-A-T-A-A-A poly(A) addition signal. The amino acid sequence derived from these nucleotides is 87% homologous to that of bovine brain calmodulin. cCM1 is one of two calmodulin genes in the chicken genome but is unique in that it does not contain intervening sequences to interrupt the structural segments of the protein. This suggests that cCM1 originated as a processed gene copy derived from the other calmodulin gene, cCL1, a circumstance usually associated with pseudogenes. In contrast, cCM1 appears to be a functional member of a multigene family whose expression is specific for muscle cells.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Calmodulin/genetics Chickens Cloning, Molecular Gene Expression Regulation Genes Species Specificity Tissue Distribution
Chemicals
Calmodulin
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Stein J P
Munjaal R P
Lagace L
Lai E C
O'Malley B W
Means A R
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32 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.80.21.6485
Published
1983-11-00
Pages
6485-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC390138
Subset
IM
Databases
GENBANK
K00510
External Links
PubMed source DOI: 10.1073/pnas.80.21.6485 PMC Full Text View journal details
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