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PMID: 23926502 Published · ppublish English Journal Article

The Profile of Human Sperm Proteome; A Mini-review.

Journal of reproduction & infertility ·Vol. 12 ·No. 3 ·2011-07-00 ·Pages 193-9

Gilany K, Lakpour N, Vafakhah M, Sadeghi MR

Abstract

New advances in mass spectrometry-based proteomics technology are having a major impact on our understanding of how human spermatozoa acquire their capacity for fertilization. A complete analysis of the proteins found in the human spermatozoa is essential for understanding the events leading up to, and including, fertilization and early embryo development. In this short review, we have collected the human sperm proteome from the literature and analyzed it by the Database for Annotation, Visualization and Integrated Discovery (DAVID) software. Bioinformatics analysis demonstrated that the collected 1,300 proteins were involved in various metabolic pathways including catabolic processes. Additionally, the majority of the collected human sperm proteome belonged to cytoplasm. Application of the multi-dimensional protein identification technology (MudPIT) for obtaining a better coverage of the hydrophobic and basic proteins of the human sperm proteome is recommended.

Keywords
DAVID bioinformatics Gene ontology Mass spectrometry-based proteomics Proteome Sperm
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Gilany Kambiz
Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran.
Lakpour Niknam
Vafakhah Mohtaram
Sadeghi Mohammad Reza
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Article Info
Journal
Journal of reproduction & infertility
Abbr.
J Reprod Infertil
ISSN
2228-5482
Published
2011-07-00
Pages
193-9
Language
English
Region
Iran
NLM ID
101535586
PMCID
PMC3719287
External Links
PubMed source PMC Full Text
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