Transforming growth factor beta 2 differentially modulates interleukin-1 beta- and tumour-necrosis-factor-alpha-stimulated phospholipase A2 and prostaglandin E2 synthesis in rat renal mesangial cells.

The Biochemical journal ·Vol. 270 ·No. 1 ·1990-08-15 ·Pages 269-71

Pfeilschifter J, Pignat W, Leighton J, Märki F, Vosbeck K, Alkan S

Abstract

Treatment of rat glomerular mesangial cells with transforming growth factor beta 2 (TGF beta 2) stimulates prostaglandin E2 (PGE2) synthesis. Actinomycin D, cycloheximide and diclofenac attenuate the TGF beta 2-induced PGE2 formation. As shown previously, two proinflammatory cytokines, interleukin 1 beta (IL-1 beta) and tumour necrosis factor alpha (TNF alpha), are potent stimuli for PGE2 and phospholipase A2 secretion from mesangial cells. We report here that, whereas TGF beta 2 potentiates the IL-1 beta- and TNF alpha-evoked PGE2 production, it strongly inhibits the phospholipase A2 secretion induced by both cytokines. In addition, the inhibitory effect of TGF beta 2 on phospholipase A2 secretion is not due to the augmented PGE2 formation.

MeSH Terms

Animals Cells, Cultured Dinoprostone/biosynthesis Dose-Response Relationship, Drug Glomerular Mesangium/metabolism In Vitro Techniques Interleukin-1/pharmacology Phospholipases/metabolism Phospholipases A/metabolism Phospholipases A2 Rats Time Factors Transforming Growth Factors/pharmacology Tumor Necrosis Factor-alpha/pharmacology

Chemicals

Interleukin-1 Tumor Necrosis Factor-alpha Transforming Growth Factors Phospholipases Phospholipases A Phospholipases A2 Dinoprostone

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Pfeilschifter J

Research Department, Ciba-Geigy Ltd., Basel, Switzerland.

Pignat W

Leighton J

Märki F

Vosbeck K

Alkan S

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Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj2700269

Published

1990-08-15

Pages

269-71

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1131710

Subset

External Links

PubMed source DOI: 10.1042/bj2700269 PMC Full Text View journal details

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Transforming growth factor beta 2 differentially modulates interleukin-1 beta- and tumour-necrosis-factor-alpha-stimulated phospholipase A2 and prostaglandin E2 synthesis in rat renal mesangial cells.

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