Home LiteratureArticle Details
PMID: 2402501 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Posttranslational modification of class III beta-tubulin.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 87 ·No. 18 ·1990-09-00 ·Pages 7195-9

Lee MK, Rebhun LI, Frankfurter A

Abstract

The charge heterogeneity of class III beta-tubulin (beta III) during neural development was analyzed by high-resolution isoelectric focusing/two-dimensional polyacrylamide gel electrophoresis in combination with site-specific proteolytic digestion and immunological detection. The number of beta III isoforms (charge variants) gradually increases from one in embryonic brain to seven in adult brain. All of the charge heterogeneity is due to posttranslationally modified sites located within the extreme C-terminal region of the beta III polypeptide. One beta III isoform is present in testis, the only other tissue in which this isotype is expressed. The testis beta III isoform cofocuses with the earliest-appearing embryonic brain beta III charge variant. Our results indicate that the posttranslational modifications of beta III are developmentally regulated, occur at more than one site, and are neuron-specific. The location of these modifications within the extreme C-terminal domain suggests that their function is to modulate the interaction of tubulin with microtubule-associated proteins.

MeSH Terms
Aging Animals Antibodies, Monoclonal Brain/embryology,growth & development,metabolism Electrophoresis, Polyacrylamide Gel Gestational Age Macromolecular Substances Male Microtubule Proteins/genetics,isolation & purification Peptide Mapping Protein Processing, Post-Translational Rats Testis/metabolism Tubulin/genetics,isolation & purification
Chemicals
Antibodies, Monoclonal Macromolecular Substances Microtubule Proteins Tubulin
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Lee M K
Department of Biology, University of Virginia, Charlottesville 22901.
Rebhun L I
Frankfurter A
References (28)
28 references, click to expand
  1. Chlamydomonas alpha-tubulin is posttranslationally modified by acetylation on the epsilon-amino group of a lysine.
    Biochemistry. 1985 Jan 15;24(2):473-8 PMID: 3919761
  2. A polymer-dependent increase in phosphorylation of beta-tubulin accompanies differentiation of a mouse neuroblastoma cell line.
    J Cell Biol. 1985 Mar;100(3):764-74 PMID: 2857724
  3. Monoclonal antibodies specific for an acetylated form of alpha-tubulin recognize the antigen in cilia and flagella from a variety of organisms.
    J Cell Biol. 1985 Dec;101(6):2085-94 PMID: 2415535
  4. Proteolysis of tubulin and the substructure of the tubulin dimer.
    J Biol Chem. 1986 Jul 5;261(19):9070-6 PMID: 3522582
  5. Biochemical and chemical properties of tubulin subspecies.
    Ann N Y Acad Sci. 1986;466:111-28 PMID: 3460411
  6. Identification of the phosphorylated beta-tubulin isotype in differentiated neuroblastoma cells.
    FEBS Lett. 1988 Mar 28;230(1-2):142-6 PMID: 3350148
  7. Complex regulation and functional versatility of mammalian alpha- and beta-tubulin isotypes during the differentiation of testis and muscle cells.
    J Cell Biol. 1988 Jun;106(6):2023-33 PMID: 3290225
  8. Tubulin structure probed with antibodies to synthetic peptides. Mapping of three major types of limited proteolysis fragments.
    Biochemistry. 1988 Jul 12;27(14):5352-65 PMID: 2458762
  9. Structure and utilization of tubulin isotypes.
    Annu Rev Cell Biol. 1988;4:687-716 PMID: 3058169
  10. Biochemical dissection of the role of the one-kilodalton carboxyl-terminal moiety of tubulin in its assembly into microtubules.
    Biochemistry. 1989 Jan 10;28(1):333-9 PMID: 2706257
  11. Posttranslational modifications of tubulin in cultured mouse brain neurons and astroglia.
    Biol Cell. 1989;65(2):109-17 PMID: 2736326
  12. The microtubule-binding fragment of microtubule-associated protein-2: location of the protease-accessible site and identification of an assembly-promoting peptide.
    J Cell Biol. 1989 Nov;109(5):2289-94 PMID: 2808529
  13. Properties of rat brain tubulin.
    J Biol Chem. 1974 Mar 10;249(5):1407-16 PMID: 4594126
  14. Microtubule assembly in the absence of added nucleotides.
    Proc Natl Acad Sci U S A. 1973 Mar;70(3):765-8 PMID: 4514990
  15. Posttranslational glutamylation of alpha-tubulin.
    Science. 1990 Jan 5;247(4938):83-5 PMID: 1967194
  16. Some common properties of the protein that incorporates tyrosine as a single unit and the microtubule proteins.
    Biochem Biophys Res Commun. 1974 Oct 23;60(4):1384-90 PMID: 4417038
  17. A protein factor essential for microtubule assembly.
    Proc Natl Acad Sci U S A. 1975 May;72(5):1858-62 PMID: 1057175
  18. An enzyme tyrosylating alpha-tubulin and its role in microtubule assembly.
    Biochem Biophys Res Commun. 1975 Aug 4;65(3):1088-95 PMID: 1156416
  19. Rat brain tubulin: subunit heterogeneity and phosphorylation.
    J Neurochem. 1979 Mar;32(3):1015-25 PMID: 430036
  20. A taxol-dependent procedure for the isolation of microtubules and microtubule-associated proteins (MAPs).
    J Cell Biol. 1982 Feb;92(2):435-42 PMID: 6120944
  21. High level of tubulin microheterogeneity in the mouse brain.
    Neurosci Lett. 1982 Aug 31;31(3):323-8 PMID: 7133567
  22. Isoelectric focusing of human IgA and secretory proteins using thin layer agarose gels and nitrocellulose capillary blotting.
    J Immunol Methods. 1984 Feb 10;66(2):313-21 PMID: 6606684
  23. Heterogeneity of vertebrate brain tubulins.
    Proc Natl Acad Sci U S A. 1984 Jul;81(13):4041-5 PMID: 6588378
  24. Involvement of the carboxyl-terminal domain of tubulin in the regulation of its assembly.
    Proc Natl Acad Sci U S A. 1984 Oct;81(19):5989-93 PMID: 6385005
  25. Controlled proteolysis of tubulin by subtilisin: localization of the site for MAP2 interaction.
    Biochemistry. 1984 Sep 25;23(20):4675-81 PMID: 6388633
  26. Programmed expression of beta-tubulin genes during development and differentiation of the chicken.
    J Cell Biol. 1984 Dec;99(6):1927-35 PMID: 6094589
  27. Tubulin subunit carboxyl termini determine polymerization efficiency.
    J Biol Chem. 1985 Jan 10;260(1):43-5 PMID: 3965457
  28. Five mouse tubulin isotypes and their regulated expression during development.
    J Cell Biol. 1985 Sep;101(3):852-61 PMID: 3839797
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.87.18.7195
Published
1990-09-00
Pages
7195-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC54710
Subset
IM
Grants
NINDS NIH HHS · NS21142 · United States
External Links
PubMed source DOI: 10.1073/pnas.87.18.7195 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2