Home LiteratureArticle Details
PMID: 2403549 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

In vivo degradation of secreted fusion proteins by the Escherichia coli outer membrane protease OmpT.

Journal of bacteriology ·Vol. 172 ·No. 1 ·1990-01-00 ·Pages 491-4

Baneyx F, Georgiou G

Abstract

The Escherichia coli outer membrane protease OmpT (protease VII) has been shown to degrade several proteins in vitro, but its function in vivo is uncertain. We demonstrate that OmpT participates in the degradation of a fusion protein secreted into the periplasmic space. A strain with mutations in degP (K.L. Strauch and J. Beckwith, Proc. Natl. Acad. Sci. USA 85:1576-1580, 1988) and ompT exhibits a cumulative decrease in protein degradation and should be useful for the expression of proteolytically sensitive secreted proteins.

MeSH Terms
Bacterial Proteins/metabolism Escherichia coli/metabolism Genes, Bacterial Recombinant Fusion Proteins/metabolism Serine Endopeptidases/physiology Staphylococcal Protein A/metabolism beta-Lactamases/metabolism
Chemicals
Bacterial Proteins Recombinant Fusion Proteins Staphylococcal Protein A Serine Endopeptidases omptin outer membrane protease beta-Lactamases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Baneyx F
Department of Chemical Engineering, University of Texas, Austin 78712.
Georgiou G
References (22)
22 references, click to expand
  1. Universal chemical assay for the detection and determination of siderophores.
    Anal Biochem. 1987 Jan;160(1):47-56 PMID: 2952030
  2. TnphoA: a transposon probe for protein export signals.
    Proc Natl Acad Sci U S A. 1985 Dec;82(23):8129-33 PMID: 2999794
  3. Complete nucleotide sequence and deduced amino acid sequence of the ompT gene of Escherichia coli K-12.
    Nucleic Acids Res. 1988 Feb 11;16(3):1209 PMID: 3278297
  4. An Escherichia coli mutation preventing degradation of abnormal periplasmic proteins.
    Proc Natl Acad Sci U S A. 1988 Mar;85(5):1576-80 PMID: 3278319
  5. Association of degradation and secretion of three chimeric polypeptides in Escherichia coli.
    J Bacteriol. 1988 May;170(5):2212-20 PMID: 3129403
  6. A novel outer-membrane-associated protease in Escherichia coli.
    J Bacteriol. 1988 Aug;170(8):3650-4 PMID: 3136144
  7. Improving the stability of a foreign protein in the periplasmic space of Escherichia coli.
    Biochimie. 1988 Jun;70(6):727-33 PMID: 3139088
  8. Purification, characterization, and primary structure of Escherichia coli protease VII with specificity for paired basic residues: identity of protease VII and OmpT.
    J Bacteriol. 1988 Dec;170(12):5625-32 PMID: 3056908
  9. Biochemical analysis of spontaneous fepA mutants of Escherichia coli.
    J Gen Microbiol. 1988 May;134(5):1355-64 PMID: 3058866
  10. Identification, characterization, and mapping of the Escherichia coli htrA gene, whose product is essential for bacterial growth only at elevated temperatures.
    J Bacteriol. 1989 Mar;171(3):1574-84 PMID: 2537822
  11. Fusion to an endoglucanase allows alkaline phosphatase to bind to cellulose.
    FEBS Lett. 1989 Feb 13;244(1):127-31 PMID: 2494059
  12. In vitro proteolytic cleavage of the Escherichia coli Ada protein by the ompT gene product.
    J Bacteriol. 1989 Apr;171(4):2249-51 PMID: 2649494
  13. Characterization of degP, a gene required for proteolysis in the cell envelope and essential for growth of Escherichia coli at high temperature.
    J Bacteriol. 1989 May;171(5):2689-96 PMID: 2540154
  14. Comparison of Escherichia coli K-12 outer membrane protease OmpT and Salmonella typhimurium E protein.
    J Bacteriol. 1989 May;171(5):2903-5 PMID: 2651422
  15. Solubilization of the cytoplasmic membrane of Escherichia coli by the ionic detergent sodium-lauryl sarcosinate.
    J Bacteriol. 1973 Sep;115(3):717-22 PMID: 4580564
  16. The release of enzymes by osmotic shock from Escherichia coli in exponential phase.
    J Biol Chem. 1966 Jul 10;241(13):3055-62 PMID: 4287907
  17. Dual affinity fusion approach and its use to express recombinant human insulin-like growth factor II.
    Proc Natl Acad Sci U S A. 1989 Jun;86(12):4367-71 PMID: 2543972
  18. Electrophoretic resolution of the "major outer membrane protein" of Escherichia coli K12 into four bands.
    FEBS Lett. 1975 Oct 15;58(1):254-8 PMID: 773686
  19. Modification of a ferric enterobactin receptor protein from the outer membrane of Escherichia coli.
    Biochem Biophys Res Commun. 1978 Jul 28;83(2):739-46 PMID: 151543
  20. omp T: Escherichia coli K-12 structural gene for protein a (3b).
    J Bacteriol. 1983 Feb;153(2):1104-6 PMID: 6337119
  21. Synthesis and assembly of the outer membrane proteins OmpA and OmpF of Escherichia coli.
    Methods Enzymol. 1983;97:112-24 PMID: 6361467
  22. ompT encodes the Escherichia coli outer membrane protease that cleaves T7 RNA polymerase during purification.
    J Bacteriol. 1988 Mar;170(3):1245-53 PMID: 3277950
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.172.1.491-494.1990
Published
1990-01-00
Pages
491-4
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC208460
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.172.1.491-494.1990 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2