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PMID: 2403551 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Interaction of monoclonal antibodies with the enzymatic domains of penicillin-binding protein 1b of Escherichia coli.

Journal of bacteriology ·Vol. 172 ·No. 1 ·1990-01-00 ·Pages 63-70

den Blaauwen T, Aarsman M, Nanninga N

Abstract

Monoclonal antibodies (MAbs) against four different antigenic determinants of penicillin-binding protein (PBP) 1b were used to study the transglycosylase and transpeptidase activities of PBP 1b. Enzyme kinetics in the presence of and without the MAbs were determined, and the synthesized murein was analyzed. Two MAbs against the transglycosylase domain of PBP 1b appeared to inhibit this reaction. One MAb inhibited only the transpeptidase reaction, and one inhibited both enzymatic activities of PBP 1b. The latter two MAbs bound to the transpeptidase domain of PBP 1b. The following major conclusions were deduced from the results. (i) Transpeptidation is the rate-limiting step of the reaction cascade, and it is dependent on the product of transglycosylation. (ii) PBP 1b has only one type of transpeptidase activity, i.e., a penta-tetra transpeptidase activity. (iii) PBP 1b is probably a globular protein which has two intimately associated enzymatic domains.

MeSH Terms
Acyltransferases/analysis,antagonists & inhibitors Antibodies, Monoclonal/immunology Bacterial Proteins Carrier Proteins Escherichia coli/analysis Hexosyltransferases/analysis,antagonists & inhibitors,immunology,metabolism Kinetics Multienzyme Complexes/analysis,immunology,metabolism Muramoylpentapeptide Carboxypeptidase Penicillin G/metabolism Penicillin-Binding Proteins Peptidoglycan/biosynthesis Peptidyl Transferases/analysis,antagonists & inhibitors,immunology,metabolism
Chemicals
Antibodies, Monoclonal Bacterial Proteins Carrier Proteins Multienzyme Complexes Penicillin-Binding Proteins Peptidoglycan Acyltransferases Peptidyl Transferases Hexosyltransferases Muramoylpentapeptide Carboxypeptidase Penicillin G
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
den Blaauwen T
Department of Molecular Cell Biology, University of Amsterdam, The Netherlands.
Aarsman M
Nanninga N
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33 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.172.1.63-70.1990
Published
1990-01-00
Pages
63-70
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC208401
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.172.1.63-70.1990 PMC Full Text View journal details
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