Home LiteratureArticle Details
PMID: 2404938 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Identification of BlaR, the signal transducer for beta-lactamase production in Bacillus licheniformis, as a penicillin-binding protein with strong homology to the OXA-2 beta-lactamase (class D) of Salmonella typhimurium.

Journal of bacteriology ·Vol. 172 ·No. 2 ·1990-02-00 ·Pages 1137-41

Zhu YF, Curran IH, Joris B, Ghuysen JM, Lampen JO

Abstract

The blaR gene of Bacillus licheniformis encodes the signal transducer for induction of the class A beta-lactamase. The protein product, BlaR, has a hydrophilic carboxy region that binds beta-lactams and shows high sequence homology to the class D beta-lactamases, particularly the OXA-2 beta-lactamase of Salmonella typhimurium. The BlaR-beta-lactam complex is stable and may provide the continuing stimulus needed for the prolonged production of the enzyme.

MeSH Terms
Amino Acid Sequence Bacillus/enzymology,genetics,metabolism Bacterial Proteins Carrier Proteins/genetics,metabolism Enzyme Induction Genes, Bacterial Genetic Vectors Hexosyltransferases Molecular Sequence Data Muramoylpentapeptide Carboxypeptidase/genetics,metabolism Penicillin-Binding Proteins Peptidyl Transferases Restriction Mapping Salmonella typhimurium/enzymology,genetics Sequence Homology, Nucleic Acid Signal Transduction beta-Lactamases/biosynthesis,genetics
Chemicals
Bacterial Proteins Carrier Proteins Penicillin-Binding Proteins Peptidyl Transferases Hexosyltransferases Muramoylpentapeptide Carboxypeptidase beta-lactamase OXA-2 beta-Lactamases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Zhu Y F
Waksman Institute, Rutgers, State University of New Jersey, Piscataway 08855-0759.
Curran I H
Joris B
Ghuysen J M
Lampen J O
References (18)
18 references, click to expand
  1. ampC cephalosporinase of Escherichia coli K-12 has a different evolutionary origin from that of beta-lactamases of the penicillinase type.
    Proc Natl Acad Sci U S A. 1981 Aug;78(8):4897-901 PMID: 6795623
  2. Penicillinase from Bacillus licheniformis: nucleotide sequence of the gene and implications for the biosynthesis of a secretory protein in a Gram-positive bacterium.
    Nucleic Acids Res. 1981 Jun 11;9(11):2577-88 PMID: 6269055
  3. A bacteriophage T7 RNA polymerase/promoter system for controlled exclusive expression of specific genes.
    Proc Natl Acad Sci U S A. 1985 Feb;82(4):1074-8 PMID: 3156376
  4. Pattern recognition in nucleic acid sequences. I. A general method for finding local homologies and symmetries.
    Nucleic Acids Res. 1982 Jan 11;10(1):247-63 PMID: 6801626
  5. Transcriptional analysis of beta-lactamase regulation in Bacillus licheniformis.
    J Bacteriol. 1986 Jun;166(3):769-78 PMID: 2423503
  6. Nucleotide sequence of the gene encoding the Streptomyces albus G beta-lactamase precursor.
    Eur J Biochem. 1987 Jul 15;166(2):345-50 PMID: 3038538
  7. A second regulatory gene, blaR1, encoding a potential penicillin-binding protein required for induction of beta-lactamase in Bacillus licheniformis.
    J Bacteriol. 1987 Sep;169(9):3873-8 PMID: 3040663
  8. The crystal structure of the beta-lactamase of Streptomyces albus G at 0.3 nm resolution.
    Biochem J. 1987 Aug 1;245(3):911-3 PMID: 3499147
  9. Precise insertion of antibiotic resistance determinants into Tn21-like transposons: nucleotide sequence of the OXA-1 beta-lactamase gene.
    Proc Natl Acad Sci U S A. 1987 Nov;84(21):7378-82 PMID: 2823258
  10. Sequence of PSE-2 beta-lactamase.
    Antimicrob Agents Chemother. 1988 Jan;32(1):134-6 PMID: 3126705
  11. The active-site-serine penicillin-recognizing enzymes as members of the Streptomyces R61 DD-peptidase family.
    Biochem J. 1988 Mar 1;250(2):313-24 PMID: 3128280
  12. The genetics of Bacillus licheniformis penicillinase: a preliminary analysis from studies on mutation and inter-strain and intra-strain transformations.
    J Gen Microbiol. 1965 Oct;41(1):7-21 PMID: 5883699
  13. Rapid fixed-time assay for penicillinase.
    J Bacteriol. 1968 Apr;95(4):1493-4 PMID: 5646633
  14. Analysis by transformation of the penicillinase system in Bacillus licheniformis.
    J Gen Microbiol. 1973 May;76(1):217-30 PMID: 4723069
  15. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  16. Penicillin-binding proteins in bacteria.
    Antimicrob Agents Chemother. 1980 Jul;18(1):148-57 PMID: 7416741
  17. The structure of beta-lactamases.
    Philos Trans R Soc Lond B Biol Sci. 1980 May 16;289(1036):321-31 PMID: 6109327
  18. Sequence of the OXA2 beta-lactamase: comparison with other penicillin-reactive enzymes.
    FEBS Lett. 1985 Oct 21;191(1):39-44 PMID: 3876949
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.172.2.1137-1141.1990
Published
1990-02-00
Pages
1137-41
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC208548
Subset
IM
Grants
NIAID NIH HHS · AI-23096 · United States
External Links
PubMed source DOI: 10.1128/jb.172.2.1137-1141.1990 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2