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PMID: 2404946 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Colicin cleavage by OmpT protease during both entry into and release from Escherichia coli cells.

Journal of bacteriology ·Vol. 172 ·No. 2 ·1990-02-00 ·Pages 648-52

Cavard D, Lazdunski C

Abstract

Proteolysis of colicins A, E1, E2, and E3 was observed after they were added to whole cells carrying a functional ompT gene. Recombinant plasmid pML19 containing the ompT gene enabled two mutant strains to cleave the added colicins. On the other hand, two colicin A recombinants were split after release from the wild-type bacteria that produced them but not from ompT mutant cells.

MeSH Terms
Colicins/isolation & purification,metabolism Electrophoresis, Polyacrylamide Gel Escherichia coli/enzymology Immunoblotting Serine Endopeptidases/metabolism
Chemicals
Colicins Serine Endopeptidases omptin outer membrane protease
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Cavard D
Centre de Biochimie et de Biologie Moléculaire, Centre National de la Recherche Scientifique, Marseille, France.
Lazdunski C
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26 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.172.2.648-652.1990
Published
1990-02-00
Pages
648-52
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC208488
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.172.2.648-652.1990 PMC Full Text View journal details
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