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PMID: 2408804 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Effect of cyclosporine A on procoagulant activity in mononuclear blood cells and monocytes in vitro.

Clinical and experimental immunology ·Vol. 60 ·No. 2 ·1985-05-00 ·Pages 407-16

Carlsen E, Mallet AC, Prydz H

Abstract

Monocytes and macrophages synthesize thromboplastin when induced by a number of compounds. Our experiments show that cyclosporine A (CyA) enhances the synthesis of procoagulant activity in monocytes and in whole mononuclear cells (MNC) about two-fold when phytohaemagglutinin (PHA) is the stimulant. The enhancement is inhibited by actinomycin D and cycloheximide. CyA alone had no effect, but in the presence of PHA or phorbol ester CyA induces or enhances the synthesis of a vitamin K-dependent procoagulant in the MNC cultures. This procoagulant acts synergistically with thromboplastin synthesized in the same cultures.

MeSH Terms
Blood Proteins/biosynthesis Cells, Cultured Cyclosporins/pharmacology Humans Leukocytes/drug effects Macrophages/drug effects,metabolism Monocytes/drug effects,metabolism Phytohemagglutinins/pharmacology Prothrombin/biosynthesis RNA/biosynthesis Stimulation, Chemical Time Factors Warfarin/pharmacology
Chemicals
Blood Proteins Cyclosporins Phytohemagglutinins Warfarin RNA Prothrombin
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Carlsen E
Mallet A C
Prydz H
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31 references, click to expand
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Article Info
Journal
Clinical and experimental immunology
Abbr.
Clin Exp Immunol
ISSN
0009-9104
Published
1985-05-00
Pages
407-16
Language
English
Region
England
NLM ID
0057202
PMCID
PMC1577035
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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