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PMID: 2409529 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Structure of the human alpha 1-acid glycoprotein gene: sequence homology with other human acute phase protein genes.

Nucleic acids research ·Vol. 13 ·No. 11 ·1985-06-11 ·Pages 3941-52

Dente L, Ciliberto G, Cortese R

Abstract

We have determined the sequence coding for human alpha 1-acid glycoprotein from two independently isolated cDNA clones and a genomic clone. The aminoacid sequences deduced from the three clones, deriving from three different individuals, are identical. Southern blot analysis on human DNA indicates that there are at least two genes coding for alpha 1-AGP. We propose that alpha 1-AGP found in plasma is a mixture of the products of these two different genes. This is the simpler explanation for the heterogeneity in the aminoacid composition in purified alpha 1-AGP observed by Schmid et al. (1). DNA sequence comparison with cDNA clones coding for human alpha 1-antitrypsin and haptoglobin shows a conserved sequence within the 5' untranslated region which may play a role in the acute phase response.

MeSH Terms
Acute-Phase Proteins Amino Acid Sequence Base Sequence Blood Proteins/genetics Cloning, Molecular Coliphages/genetics DNA Restriction Enzymes Escherichia coli/genetics Genes Haptoglobins/genetics Humans Nucleic Acid Hybridization Orosomucoid/genetics Plasmids RNA, Messenger/genetics Structure-Activity Relationship alpha 1-Antitrypsin/genetics
Chemicals
Acute-Phase Proteins Blood Proteins Haptoglobins Orosomucoid RNA, Messenger alpha 1-Antitrypsin DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Dente L
Ciliberto G
Cortese R
References (27)
27 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/13.11.3941
Published
1985-06-11
Pages
3941-52
Language
English
Region
England
NLM ID
0411011
PMCID
PMC341288
Subset
IM
Databases
GENBANK
X02544
External Links
PubMed source DOI: 10.1093/nar/13.11.3941 PMC Full Text View journal details
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