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PMID: 2410904 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

The sequence of a type II keratin gene expressed in human skin: conservation of structure among all intermediate filament genes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 14 ·1985-07-00 ·Pages 4683-7

Tyner AL, Eichman MJ, Fuchs E

Abstract

We report here the coding sequence of the gene for a 56-kDa type II keratin (designated K6b). Using a subclone specific for a unique 3' noncoding region of the encoded mRNA, we have shown that this gene is one of at least two 56-kDa keratin genes expressed in abundance in human epidermis. Segmenting the coding portion of this gene are eight introns, six of which are identically positioned with those of a distantly related type III intermediate filament gene (vimentin), and five of which are identically positioned with those of a distantly related type I gene (50-kDa keratin). These results indicate a common ancestral origin for all three classes of intermediate filament genes. All of the highly conserved intron positions are located within, but do not demarcate, the four central alpha-helical domains common to all intermediate filament polypeptides, suggesting that these genes were probably not created piecemeal by recombination-mediated linkage of separate structural domains as they presently are known.

MeSH Terms
Amino Acid Sequence Base Sequence Cloning, Molecular Genes Humans Intermediate Filament Proteins/genetics Keratins/genetics Nucleic Acid Hybridization Plasmids RNA, Messenger/genetics Skin/metabolism Transcription, Genetic
Chemicals
Intermediate Filament Proteins RNA, Messenger Keratins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Tyner A L
Eichman M J
Fuchs E
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37 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.82.14.4683
Published
1985-07-00
Pages
4683-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC390450
Subset
IM
Grants
NIADDK NIH HHS · R01-AM 27883 · United States
Databases
GENBANK
L00198, L00199, L00200, L00201, L00202, L00203, L00204, L00205, M11229
External Links
PubMed source DOI: 10.1073/pnas.82.14.4683 PMC Full Text View journal details
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