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PMID: 2410918 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Assignment of the gene for human DNA polymerase alpha to the X chromosome.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 16 ·1985-08-00 ·Pages 5270-4

Wang TS, Pearson BE, Suomalainen HA, Mohandas T, Shapiro LJ, Schröder J, Korn D

Abstract

We have applied an assay based on a monoclonal antibody that discriminates the activity of human DNA polymerase alpha in rodent-human somatic cell hybrid clones to identify a single genetic locus that is both necessary and sufficient for the expression of DNA polymerase alpha. We have mapped this locus to the short arm of the human X chromosome, near the junction of bands Xp21.3 and Xp22.1, and demonstrated that it is not expressed from an inactive X chromosome.

MeSH Terms
Animals Antibodies, Monoclonal Cell Line Chromosome Mapping Clone Cells DNA Polymerase II/genetics Epitopes/analysis Female Genes Humans Hybrid Cells/enzymology Hypoxanthine Phosphoribosyltransferase/deficiency Lymphocytes/enzymology Mice Species Specificity X Chromosome
Chemicals
Antibodies, Monoclonal Epitopes Hypoxanthine Phosphoribosyltransferase DNA Polymerase II
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Wang T S
Pearson B E
Suomalainen H A
Mohandas T
Shapiro L J
Schröder J
Korn D
References (23)
23 references, click to expand
  1. A rapid banding technique for human chromosomes.
    Lancet. 1971 Oct 30;2(7731):971-2 PMID: 4107917
  2. The cell surface antigen locus, MIC2X, escapes X-inactivation.
    Am J Hum Genet. 1984 Jul;36(4):777-82 PMID: 6540985
  3. Human hypoxanthine-guanine phosphoribosyltransferase. Purification and subunit structure.
    J Biol Chem. 1971 Dec 10;246(23):7398-404 PMID: 5129729
  4. Mechanisms of giemsa banding of chromosomes. I. Giemsa-11 banding with azure and eosin.
    Exp Cell Res. 1976 Oct 1;102(1):85-94 PMID: 61884
  5. Determination of nucleic acid sequence homologies and relative concentrations by a dot hybridization procedure.
    Nucleic Acids Res. 1979 Nov 24;7(6):1541-52 PMID: 503860
  6. Regional assignment of the steroid sulfatase-X-linked ichthyosis locus: implications for a noninactivated region on the short arm of human X chromosome.
    Proc Natl Acad Sci U S A. 1979 Nov;76(11):5779-83 PMID: 293682
  7. Localization of loci for hypoxanthine phosphoribosyltransferase and glucose-6-phosphate dehydrogenase and biochemical evidence of nonrandom X chromosome expression from studies of a human X-autosome translocation.
    Proc Natl Acad Sci U S A. 1980 May;77(5):2810-3 PMID: 6930669
  8. Human ornithine transcarbamylase locus mapped to band Xp21.1 near the Duchenne muscular dystrophy locus.
    Science. 1984 Nov 9;226(4675):698-700 PMID: 6494904
  9. Random X inactivation resulting in mosaic nullisomy of region Xp21.1----p21.3 associated with heterozygosity for ornithine transcarbamylase deficiency and for chronic granulomatous disease.
    Cytogenet Cell Genet. 1984;38(4):298-307 PMID: 6510024
  10. Mouse/human T-cell hybrids rosetting with sheep erythrocytes.
    Scand J Immunol. 1980;11(2):163-8 PMID: 9537042
  11. An (X;11) translocation in a girl with Duchenne muscular dystrophy. Repository identification No. GM1695.
    Cytogenet Cell Genet. 1980;27(4):268 PMID: 7438786
  12. A human X-linked antigen defined by a monoclonal antibody.
    Somatic Cell Genet. 1980 Nov;6(6):777-87 PMID: 6969453
  13. Expression of an X-linked gene from an inactive human X chromosome in mouse-human hybrid cells: further evidence for the noninactivation of the steroid sulfatase locus in man.
    Proc Natl Acad Sci U S A. 1980 Nov;77(11):6759-63 PMID: 6935682
  14. Reactivation of an inactive human X chromosome: evidence for X inactivation by DNA methylation.
    Science. 1981 Jan 23;211(4480):393-6 PMID: 6164095
  15. Structural analysis of templates and RNA polymerase III transcripts of Alu family sequences interspersed among the human beta-like globin genes.
    Gene. 1981 Mar;13(2):185-96 PMID: 6165649
  16. Preparation and preliminary characterization of monoclonal antibodies against human DNA polymerase alpha.
    J Biol Chem. 1982 Jul 25;257(14):8386-90 PMID: 7085672
  17. Genetic evidence for the inactivation of a human autosomal locus attached to an inactive X chromosome.
    Am J Hum Genet. 1982 Sep;34(5):811-7 PMID: 7124733
  18. Fluorescence-activated cell sorting of mouse-human hybrid cells aids in locating the gene for the Leu 7 (HNK-1) antigen to human chromosome 11.
    Proc Natl Acad Sci U S A. 1983 Jun;80(11):3421-4 PMID: 6344083
  19. Report of the Committee on the Genetic Constitution of the X and Y Chromosomes.
    Cytogenet Cell Genet. 1984;37(1-4):176-204 PMID: 6360558
  20. DNA primase from KB cells. Characterization of a primase activity tightly associated with immunoaffinity-purified DNA polymerase-alpha.
    J Biol Chem. 1984 Feb 10;259(3):1854-65 PMID: 6693436
  21. DNA primase from KB cells. Evidence for a novel model of primase catalysis by a highly purified primase/polymerase-alpha complex.
    J Biol Chem. 1984 Feb 25;259(4):2602-9 PMID: 6698983
  22. Isolation and characterization of human factor IX cDNA: identification of Taq I polymorphism and regional assignment.
    Somat Cell Mol Genet. 1984 Sep;10(5):465-73 PMID: 6089357
  23. Enzyme electrophoresis on cellulose acetate gel: zymogram patterns in mgh-mouse and man--Chinese hamster somatic cell hybrids.
    Arch Biochem Biophys. 1971 Aug;145(2):470-83 PMID: 4331030
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.82.16.5270
Published
1985-08-00
Pages
5270-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC390549
Subset
IM
Grants
NCI NIH HHS · CA-14835 · United States
External Links
PubMed source DOI: 10.1073/pnas.82.16.5270 PMC Full Text View journal details
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