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PMID: 2411537 Published · ppublish English Journal Article

Levels of nerve growth factor and its mRNA in the central nervous system of the rat correlate with cholinergic innervation.

The EMBO journal ·Vol. 4 ·No. 6 ·1985-06-00 ·Pages 1389-93

Korsching S, Auburger G, Heumann R, Scott J, Thoenen H

Abstract

The levels of nerve growth factor (NGF) and its mRNA in the rat central nervous system were determined by two-site enzyme immunoassay and quantitative Northern blots, respectively. Relatively high NGF levels (0.4-1.4 ng NGF/g wet weight) were found both in the regions innervated by the magnocellular cholinergic neurons of the basal forebrain (hippocampus, olfactory bulb, neocortex) and in the regions containing the cell bodies of these neurons (septum, nucleus of the diagonal band of Broca, nucleus basalis of Meynert). Comparatively low, but significant NGF levels (0.07-0.21 ng NGF/g wet weight) were found in various other brain regions. mRNANGF was found in the hippocampus and cortex but not in the septum. This suggests that magnocellular cholinergic neurons of the basal forebrain are supplied with NGF via retrograde axonal transport from their fields of innervation. These results, taken together with those of previous studies showing that these neurons are responsive to NGF, support the concept that NGF acts as trophic factor for magnocellular cholinergic neurons.

MeSH Terms
Animals Axonal Transport Brain Chemistry Dissection Nerve Growth Factors/analysis,genetics Parasympathetic Nervous System/analysis RNA, Messenger/analysis Rats Rats, Inbred Strains Sympathectomy Time Factors
Chemicals
Nerve Growth Factors RNA, Messenger
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Korsching S
Auburger G
Heumann R
Scott J
Thoenen H
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36 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1985-06-00
Pages
1389-93
Language
English
Region
England
NLM ID
8208664
PMCID
PMC554356
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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