Mitochondrial DNA from Podospora anserina : IV. The large ribosomal RNA gene contains two long intervening sequences.

Current genetics ·Vol. 7 ·No. 2 ·1983-04-00 ·Pages 151-7

Wright RM, Cummings DJ

Abstract

We have examined the structure of the rRNA genes from the mitochondrial genome of Podospora anserina. Using R-loop analysis, nuclease protection experiments, and Southern blot hybridization analysis we have observed two intervening sequences (IVS) in the large rRNA gene, and none in the small rRNA gene. the IVS sequences are 1.65 kbp and 2.73 kbp long, and the larger of the two is in the position of the conserved IVS found in the mitochondrial genomes of other fungi. We have detected precursor transcripts for the large rRNA, and these data support the observation of two IVS in this gene. We also note that the large and small rRNA genes are separated by approximately 6 kbp of DNA.

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Wright R M

Department of Microbiology and Immunology, School of Medicine, University of Colorado, Health Sciences Center, Campus Box B-175, 80262, Denver, Colorado, USA.

Cummings D J

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Article Info

Journal

Current genetics

Abbr.

Curr Genet

ISSN

0172-8083

DOI

10.1007/BF00365641

Published

1983-04-00

Pages

151-7

Language

English

Region

United States

NLM ID

8004904

External Links

PubMed source DOI: 10.1007/BF00365641 View journal details

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Mitochondrial DNA from Podospora anserina : IV. The large ribosomal RNA gene contains two long intervening sequences.

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