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PMID: 286867 Published · ppublish English Journal Article

Mitochondrial DNA from Podospora anserina. I. Isolation and characterization.

Molecular & general genetics : MGG ·Vol. 171 ·No. 3 ·1979-03-27 ·Pages 229-38

Cummings DJ, Belcour L, Grandchamp C

Abstract

Mitochondrial (Mt) DNA from Podospora anserina was isolated and characterized with respect to density in CsCl, contour length and endonuclease restriction enzymes. The density of Mt DNA for four races examined was 1.694 g/cm3, compared with 1.712 g/cm3 for nuclear DNA. Extraction in the presence of a nuclease inhibitor, aurintricarboxylic acid and isolation in DAPI CsCl gradients allowed us to isolate high molecular weight DNA. Mt DNA isolated by total DNA extraction contained ca. 1% of circular molecules, 31 micron in contour length; Mt DNA isolated from purified mitochondria contained 2--4% of these 31 micron circles. Analysis with Eco RI restriction endonuclease revealed that each of the four races examined, s, A, T and E had a characteristic fragment pattern. Races s and A Mt DNA differed by only one fragment after Eco RI enzymatic digestion; similarly, these two DNA differed by only one or two fragments after Hae III digestion.

MeSH Terms
Ascomycota/genetics Centrifugation, Density Gradient DNA Restriction Enzymes/metabolism DNA, Mitochondrial/isolation & purification Microscopy, Electron Molecular Weight Nucleic Acid Conformation
Chemicals
DNA, Mitochondrial DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Cummings D J
Belcour L
Grandchamp C
References (36)
36 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00267577
Published
1979-03-27
Pages
229-38
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00267577
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