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PMID: 410006 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Use of aurintricarboxylic acid as an inhibitor of nucleases during nucleic acid isolation.

Nucleic acids research ·Vol. 4 ·No. 9 ·1977-09-00 ·Pages 3055-64

Hallick RB, Chelm BK, Gray PW, Orozco EM

Abstract

Aurintricarboxylic acid (ATA) is a general inhibitor of nucleases. ATA has been shown to inhibit the following enzymes in vitro: DNAse I, RNAse A, S1 nuclease, exonuclease III, and restriction endonucleases Sal I, Bam HI, Pst I and Sma I. The observed inhibition is consistent with the proposal by Blumenthal and Landers (BBRC 55, 680, 1973) that most nucleic acid binding proteins will be sensitive to ATA. The action of ATA as a nuclease inhibitor can be used to advantage in the isolation of cellular nucleic acids.

MeSH Terms
Aurintricarboxylic Acid/pharmacology Chloroplasts/analysis Cyclohexanecarboxylic Acids/pharmacology DNA/isolation & purification DNA Restriction Enzymes/antagonists & inhibitors Deoxyribonucleases/antagonists & inhibitors Euglena gracilis Kinetics Methods RNA/isolation & purification Ribonucleases/antagonists & inhibitors
Chemicals
Cyclohexanecarboxylic Acids Aurintricarboxylic Acid RNA DNA Deoxyribonucleases Ribonucleases DNA Restriction Enzymes
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Hallick R B
Chelm B K
Gray P W
Orozco E M
References (23)
23 references, click to expand
  1. Crystalline desoxyribonuclease; isolation and general properties; spectrophotometric method for the measurement of desoxyribonuclease activity.
    J Gen Physiol. 1950 Mar;33(4):349-62 PMID: 15406373
  2. DEOXYRIBONUCLEIC ACID FROM THE CHLOROPLASTS OF EUGLENA GRACILIS.
    Biochim Biophys Acta. 1964 Nov 15;91:477-85 PMID: 14254019
  3. [Some factors which affect the enzymatic digestion of ribonucleic acid].
    J Biol Chem. 1959 Jun;234(6):1512-6 PMID: 13654407
  4. Characterization of the nucleoside triphosphate phosphohydrolase (ATPase) activity of RNA synthesi termination factor p. I. Enzymatic properties and effects of inhibitors.
    J Biol Chem. 1977 Feb 25;252(4):1375-80 PMID: 138681
  5. Restriction endonuclease map of Euglena gracilis chloroplast DNA.
    Biochemistry. 1977 Apr 19;16(8):1665-71 PMID: 403933
  6. Restriction endonucleases.
    CRC Crit Rev Biochem. 1976 Nov;4(2):123-64 PMID: 795607
  7. Effects of aurintricarboxylic acid on ribosomes and the biosynthesis of globin in rabbit reticulocytes.
    Mol Pharmacol. 1972 Mar;8(2):111-27 PMID: 5025198
  8. Aurintricarboxylic acid: inhibitor of initiation of protein synthesis.
    Proc Natl Acad Sci U S A. 1971 Jan;68(1):97-101 PMID: 5276307
  9. The inhibition of nucleic acid-binding proteins by aurintricarboxylic acid.
    Biochem Biophys Res Commun. 1973 Dec 10;55(3):680-8 PMID: 4586617
  10. Large subunit ribulosebisphosphate carboxylase messenger RNA from Euglena chloroplasts.
    Proc Natl Acad Sci U S A. 1976 Mar;73(3):722-6 PMID: 815906
  11. Isolation of a transcriptionally active chromosome from chloroplasts of Euglena gracilis.
    Biochemistry. 1976 Jul 13;15(14):3039-45 PMID: 821516
  12. Inhibition of RNA-directed DNA polymerase by aurintricarboxylic acid.
    Nucleic Acids Res. 1976 Feb;3(2):405-18 PMID: 56743
  13. Fate of the message-ribosome complex upon translation of termination signals.
    J Mol Biol. 1969 Jun 28;42(3):425-39 PMID: 4896187
  14. Studies on the cleavage of bacteriophage lambda DNA with EcoRI Restriction endonuclease.
    J Mol Biol. 1975 Jan 25;91(3):315-28 PMID: 1102702
  15. The isolation and partial characterization of a new restriction endonuclease from Providencia stuartii.
    Nucleic Acids Res. 1976 Feb;3(2):343-53 PMID: 768923
  16. Aurintricarboxylic acid is a nonspecific enzyme inhibitor.
    Mol Pharmacol. 1976 Jan;12(1):191-3 PMID: 1256444
  17. Changes in the expression of the chloroplast genome of Euglena gracilis during chloroplast development.
    Biochemistry. 1976 Feb 10;15(3):593-9 PMID: 1252414
  18. Preparation and preliminary characterization of purified ovalbumin messenger RNA from the hen oviduct.
    Biochemistry. 1975 Jan 14;14(1):69-78 PMID: 1109593
  19. Inhibition of the attachment of messenger ribonucleic acid to ribosomes.
    Proc Natl Acad Sci U S A. 1968 Oct;61(2):719-25 PMID: 5246002
  20. A crude nuclease preparation suitable for use in DNA reassociation experiments.
    Biochim Biophys Acta. 1971 Jul 29;240(4):522-31 PMID: 5123563
  21. Aurintricarboxylic acid, a preferential inhibitor of initiation of protein synthesis.
    J Bacteriol. 1971 Mar;105(3):902-7 PMID: 5547994
  22. Separation and characterization of Euglena gracilis chloroplast single-strand DNA.
    Biochim Biophys Acta. 1970 May 21;209(1):16-23 PMID: 5421962
  23. Evidence for the direction of chloroplasts ribosomal RNA synthesis by chloroplast DNA.
    Biochem Biophys Res Commun. 1967 Aug 23;28(4):598-603 PMID: 6059238
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/4.9.3055
Published
1977-09-00
Pages
3055-64
Language
English
Region
England
NLM ID
0411011
PMCID
PMC342634
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/4.9.3055 PMC Full Text View journal details
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