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PMID: 24177937 Published · ppublish English Journal Article

Extension of the linkage map in Citrus using random amplified polymorphic DNA (RAPD) markers and RFLP mapping of cold-acclimation-responsive loci.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik ·Vol. 89 ·No. 5 ·1994-11-00 ·Pages 606-14

Cai Q, Guy CL, Moore GA

Abstract

Genetic mapping with RAPD markers has been initiated in Citrus. Reproducible polymorphism of amplified DNA fragments was obtained with approximately half of the 140 random primers tested, revealing 266 segregating loci. These were tested for linkage using 60 BC1 progeny from an intergeneric cross of Citrus grandis (L.) Osb. x [Citrus grandis (L.) Osb. x Poncirus trifoliata (L.) Raf.]. A core linkage map was constructed that consists of nine linkage groups containing 109 RAPD markers and 51 previously-mapped RFLP and isozyme markers. A further 79 markers that could not be ordered unambiguously because of their allelic constitution were associated with individual linkage groups and are shown in relation to the core map. The core map has a total length of 1192 cM with an average distance of 7.5 cM between loci and is estimated to cover 70-80% of the genome. Loci with distorted segregation patterns clustered on several linkage groups. Individual clusters of loci were skewed in allelic composition toward one or the other parent, usually C. grandis. This relatively-saturated linkage map will eventually be used to identify quantitative trait loci for cold and salt-tolerance in Citrus. As a beginning we have mapped three loci detected by a cold-acclimation-responsive cDNA.

Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Cai Q
Plant Molecular and Cellular Biology Program, Departments of Environmental and Horticultural Sciences, Institute of Food and Agricultural Sciences, University of Florida, 32611, Gainesville, FL, USA.
Guy C L
Moore G A
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Article Info
Journal
TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik
Abbr.
Theor Appl Genet
ISSN
0040-5752
DOI
10.1007/BF00222455
Published
1994-11-00
Pages
606-14
Language
English
Region
Germany
NLM ID
0145600
External Links
PubMed source DOI: 10.1007/BF00222455 View journal details
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