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PMID: 2418214 Published · ppublish English Comparative Study Journal Article

Murine mammary tumor virus pol-related sequences in human DNA: characterization and sequence comparison with the complete murine mammary tumor virus pol gene.

Journal of virology ·Vol. 57 ·No. 2 ·1986-02-00 ·Pages 422-32

Deen KC, Sweet RW

Abstract

Sequences in the human genome with homology to the murine mammary tumor virus (MMTV) pol gene were isolated from a human phage library. Ten clones with extensive pol homology were shown to define five separate loci. These loci share common sequences immediately adjacent to the pol-like segments and, in addition, contain a related repeat element which bounds this region. This organization is suggestive of a proviral structure. We estimate that the human genome contains 30 to 40 copies of these pol-related sequences. The pol region of one of the cloned segments (HM16) and the complete MMTV pol gene were sequenced and compared. The nucleotide homology between these pol sequences is 52% and is concentrated in the terminal regions. The MMTV pol gene contains a single long open reading frame encoding 899 amino acids and is demarcated from the partially overlapping putative gag gene by termination codons and a shift in translational reading frame. The pol sequence of HM16 is multiply terminated but does contain open reading frames which encode 370, 105, and 112 amino acid residues in separate reading frames. We deduced a composite pol protein sequence for HM16 by aligning it to the MMTV pol gene and then compared these sequences with other retroviral pol protein sequences. Conserved sequences occur in both the amino and carboxyl regions which lie within the polymerase and endonuclease domains of pol, respectively.

MeSH Terms
Amino Acid Sequence Base Sequence Genes Genes, Viral Humans Mammary Tumor Virus, Mouse/genetics RNA-Directed DNA Polymerase/genetics Repetitive Sequences, Nucleic Acid Sequence Homology, Nucleic Acid
Chemicals
RNA-Directed DNA Polymerase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Deen K C
Sweet R W
References (51)
51 references, click to expand
  1. Further evidence for the protein coding potential of the mouse mammary tumor virus long terminal repeat: nucleotide sequence of an endogenous proviral long terminal repeat.
    J Virol. 1983 Mar;45(3):941-9 PMID: 6300464
  2. Nucleotide sequence of Rous sarcoma virus.
    Cell. 1983 Mar;32(3):853-69 PMID: 6299578
  3. Human adult T-cell leukemia virus: complete nucleotide sequence of the provirus genome integrated in leukemia cell DNA.
    Proc Natl Acad Sci U S A. 1983 Jun;80(12):3618-22 PMID: 6304725
  4. Mouse mammary tumour virus related sequences are present in human DNA.
    Nucleic Acids Res. 1983 Jun 25;11(12):4127-39 PMID: 6306576
  5. Nucleotide sequencing of an apparent proviral copy of env mRNA defines determinants of expression of the mouse mammary tumor virus env gene.
    J Virol. 1983 Sep;47(3):495-504 PMID: 6312081
  6. Sequence homology between retroviral reverse transcriptase and putative polymerases of hepatitis B virus and cauliflower mosaic virus.
    Nature. 1983 Oct 27-Nov 2;305(5937):827-9 PMID: 6195530
  7. Nucleotide sequence of the 5' noncoding region and part of the gag gene of mouse mammary tumor virus; identification of the 5' splicing site for subgenomic mRNAs.
    Nucleic Acids Res. 1983 Oct 25;11(20):6943-55 PMID: 6314267
  8. mRNA transcripts related to full-length endogenous retroviral DNA in human cells.
    Nature. 1983 Dec 8-14;306(5943):604-7 PMID: 6646236
  9. Major pol gene progenitors in the evolution of oncoviruses.
    Science. 1984 Jan 27;223(4634):364-70 PMID: 6197754
  10. Murine leukemia virus mutant with a frameshift in the reverse transcriptase coding region: implications for pol gene structure.
    J Virol. 1984 Aug;51(2):470-8 PMID: 6205170
  11. Distinctive termini characterize two families of human endogenous retroviral sequences.
    Science. 1984 Aug 31;225(4665):943-7 PMID: 6089336
  12. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  13. Establishment and characterization of a cell line of human breast carcinoma origin.
    Eur J Cancer. 1979 May;15(5):659-70 PMID: 228940
  14. Requirement of the avian retrovirus pp32 DNA binding protein domain for replication.
    Virology. 1984 Sep;137(2):358-70 PMID: 6091334
  15. ERV3, a full-length human endogenous provirus: chromosomal localization and evolutionary relationships.
    Virology. 1984 Oct 30;138(2):225-35 PMID: 6495650
  16. Identification of the coding sequence for a reverse transcriptase-like enzyme in a transposable genetic element in Drosophila melanogaster.
    Nature. 1984 Dec 13-19;312(5995):659-61 PMID: 6209583
  17. The long terminal repeat sequences of a novel human endogenous retrovirus.
    Science. 1984 Dec 7;226(4679):1204-6 PMID: 6505687
  18. Nucleotide sequence of the AIDS virus, LAV.
    Cell. 1985 Jan;40(1):9-17 PMID: 2981635
  19. Complete nucleotide sequence of the AIDS virus, HTLV-III.
    Nature. 1985 Jan 24-30;313(6000):277-84 PMID: 2578615
  20. Retroviruses and retrotransposons: the role of reverse transcription in shaping the eukaryotic genome.
    Cell. 1985 Mar;40(3):481-2 PMID: 2578883
  21. Rous sarcoma virus encodes a transcriptional activator.
    Cell. 1985 Mar;40(3):537-46 PMID: 2982497
  22. Complete nucleotide sequence of the genome of bovine leukemia virus: its evolutionary relationship to other retroviruses.
    Proc Natl Acad Sci U S A. 1985 Feb;82(3):677-81 PMID: 2983308
  23. Murine leukemia virus protease is encoded by the gag-pol gene and is synthesized through suppression of an amber termination codon.
    Proc Natl Acad Sci U S A. 1985 Mar;82(6):1618-22 PMID: 3885215
  24. Reverse transcriptase rides again.
    Nature. 1985 Apr 18-24;314(6012):583-4 PMID: 2581138
  25. Nucleotide sequence of a full-length human endogenous retroviral segment.
    J Virol. 1985 Jun;54(3):764-72 PMID: 3999194
  26. A new class of endogenous human retroviral genomes.
    Science. 1985 Jun 7;228(4704):1208-11 PMID: 2408338
  27. Sequence and expression of the mouse mammary tumour virus env gene.
    EMBO J. 1983;2(1):125-31 PMID: 11894899
  28. A human cell line from a pleural effusion derived from a breast carcinoma.
    J Natl Cancer Inst. 1973 Nov;51(5):1409-16 PMID: 4357757
  29. Isolation and characterization of RNA-directed DNA polymerase from a B-type RNA tumor virus.
    J Virol. 1974 Jul;14(1):40-6 PMID: 4134465
  30. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  31. Purification and properties of murine mammary tumor virus DNA polymerase.
    Virology. 1976 May;71(1):242-54 PMID: 58470
  32. Screening lambdagt recombinant clones by hybridization to single plaques in situ.
    Science. 1977 Apr 8;196(4286):180-2 PMID: 322279
  33. Continuous growth and differentiation of human myeloid leukaemic cells in suspension culture.
    Nature. 1977 Nov 24;270(5635):347-9 PMID: 271272
  34. Reverse transcriptase of RNA tumor viruses. V. In vitro proteolysis of reverse transcriptase from avian myeloblastosis virus and isolation of a polypeptide manifesting only RNase H activity.
    J Virol. 1978 Feb;25(2):652-63 PMID: 75271
  35. The isolation and characterization of linked delta- and beta-globin genes from a cloned library of human DNA.
    Cell. 1978 Dec;15(4):1157-74 PMID: 728996
  36. Purification and properties of RNA-dependent DNA polymerase from cytoplasmic A-type particles of murine mammary tumor virus.
    Eur J Biochem. 1979 Jun;97(1):257-66 PMID: 89959
  37. Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate.
    Proc Natl Acad Sci U S A. 1979 Aug;76(8):3683-7 PMID: 291033
  38. Protein-coding potential of mouse mammary tumor virus genome RNA as examined by in vitro translation.
    J Virol. 1981 Jan;37(1):36-47 PMID: 6260988
  39. Hormone-responsive expression of an endogenous proviral gene of mouse mammary tumor virus after molecular cloning and gene transfer into cultured cells.
    Proc Natl Acad Sci U S A. 1981 Apr;78(4):2038-42 PMID: 6264458
  40. Frameshift suppression.
    Cell. 1981 Jun;24(3):601-2 PMID: 6166384
  41. Purification and translation of murine mammary tumor virus mRNA's.
    J Virol. 1981 Jul;39(1):207-18 PMID: 6268810
  42. Nucleotide sequence of Moloney murine leukaemia virus.
    Nature. 1981 Oct 15-21;293(5833):543-8 PMID: 6169994
  43. Identification and cloning of endogenous retroviral sequences present in human DNA.
    Proc Natl Acad Sci U S A. 1981 Aug;78(8):4892-6 PMID: 6272283
  44. Los Alamos sequence analysis package for nucleic acids and proteins.
    Nucleic Acids Res. 1982 Jan 11;10(1):183-96 PMID: 6174934
  45. Retrovirus-related sequences in human DNA: detection and cloning of sequences which hybridize with the long terminal repeat of baboon endogenous virus.
    Nucleic Acids Res. 1982 May 11;10(9):2865-78 PMID: 6285313
  46. Cloned endogenous retroviral sequences from human DNA.
    Proc Natl Acad Sci U S A. 1982 Aug;79(15):4709-13 PMID: 6181510
  47. Detection and cloning of human DNA sequences related to the mouse mammary tumor virus genome.
    Proc Natl Acad Sci U S A. 1982 Sep;79(18):5503-7 PMID: 6291047
  48. Conversion of RNA to DNA in mammals: Alu-like elements and pseudogenes.
    Nature. 1983 Feb 10;301(5900):471-2 PMID: 6185852
  49. Characterization and partial nucleotide sequence of endogenous type C retrovirus segments in human chromosomal DNA.
    Proc Natl Acad Sci U S A. 1983 Feb;80(3):678-82 PMID: 6298769
  50. Rapid similarity searches of nucleic acid and protein data banks.
    Proc Natl Acad Sci U S A. 1983 Feb;80(3):726-30 PMID: 6572363
  51. Mapping of an endogenous retroviral sequence to human chromosome 18.
    Nature. 1983 May 5-11;303(5912):74-7 PMID: 6843662
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.57.2.422-432.1986
Published
1986-02-00
Pages
422-32
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC252753
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.57.2.422-432.1986 PMC Full Text View journal details
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