Structure of the genomic DNA encoding cucumber ascorbate oxidase and its expression in transgenic plants.
Genomic DNA (Aso1) encoding the ascorbate oxidase (ASO) of cucumber (Cucumis sativas) was cloned and sequenced. From a comparison of the sequences of Aso1 and its cDNA [Ohkawa et al. Proc. Natl. Acad. Sci. USA 86 (1989) 1239-1243], it was concluded that Aso1 consisted of four exons and three introns. In the 5'-flanking region, sequence motif resembling a TATA box and a CAAT box, were found 124 and 218 bp upstream, respectively, from the start codon. Sites of initiation of transcription, determined by primer extension, were the A and G residues located 90 and 93 bp, respectively, upstream from the initiation codon. ASO was abundant in cucumber fruits and stems. The 5'-flanking region, placed upstream of the structural gene of β-glucuronidase (GUS), expressed the GUS activity in cucumber plants in a transient expression assay after introduction of DNA with a particle gun. High-level GUS expression was found in stems of transgenic tobacco plants harboring Aso1 promoter-GUS chimeric gene.
16 references, click to expand
-
A general method for isolation of high molecular weight DNA from eukaryotes.Nucleic Acids Res. 1976 Sep;3(9):2303-8 PMID: 987581
-
X-ray crystal structure of the blue oxidase ascorbate oxidase from zucchini. Analysis of the polypeptide fold and a model of the copper sites and ligands.J Mol Biol. 1989 Apr 5;206(3):513-29 PMID: 2716059
-
The effects of copper nutrition and developmental state on the biosynthesis of diamine oxidase in clover leaves.Plant Physiol. 1986 Dec;82(4):1126-31 PMID: 16665146
-
Cloning, sequence analysis, and expression of ligninolytic phenoloxidase genes of the white-rot basidiomycete Coriolus hirsutus.J Biol Chem. 1990 Sep 5;265(25):15224-30 PMID: 2394718
-
Expression of Ascorbic Acid Oxidase in Zucchini Squash (Cucurbita pepo L.).Plant Physiol. 1991 May;96(1):159-65 PMID: 16668145
-
New M13 vectors for cloning.Methods Enzymol. 1983;101:20-78 PMID: 6310323
-
Characterization of two allelic forms of Neurospora crassa laccase. Amino- and carboxyl-terminal processing of a precursor.J Biol Chem. 1988 Jan 15;263(2):885-96 PMID: 2961749
-
Rapid sequence determination of late simian virus 40 16S mRNA leader by using inhibitors of reverse transcriptase.Proc Natl Acad Sci U S A. 1979 Feb;76(2):731-5 PMID: 85304
-
DNA sequencing with chain-terminating inhibitors.Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
-
The blue oxidases, ascorbate oxidase, laccase and ceruloplasmin. Modelling and structural relationships.Eur J Biochem. 1990 Jan 26;187(2):341-52 PMID: 2404764
-
Rapid degradation of apoplastocyanin in Cu(II)-deficient cells of Chlamydomonas reinhardtii.J Biol Chem. 1986 Dec 5;261(34):15850-3 PMID: 3023330
-
Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing.Gene. 1984 Jun;28(3):351-9 PMID: 6235151
-
In vitro packaging of lambda and cosmid DNA.Methods Enzymol. 1979;68:299-309 PMID: 161604
-
Point mutations close to the AUG initiator codon affect the efficiency of translation of rat preproinsulin in vivo.Nature. 1984 Mar 15-21;308(5956):241-6 PMID: 6700727
-
GUS fusions: beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants.EMBO J. 1987 Dec 20;6(13):3901-7 PMID: 3327686
-
Structure and expression of the gene coding for a multicopper enzyme, ascorbate oxidase of cucumber.Ann N Y Acad Sci. 1990;613:483-8 PMID: 2075998
Contact
No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong
Qilu Normal University · Genelibs Bioinformatics Lab
750 Shunhua Rd, Jinan
2F, Bldg F, University Science Park
Tel: 0531-88819269
WeChat Official Account
Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.
Business Email
E-mail: [email protected]
Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2
