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PMID: 2420781 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Rho-dependent transcription termination in the tryptophanase operon leader region of Escherichia coli K-12.

Journal of bacteriology ·Vol. 166 ·No. 1 ·1986-04-00 ·Pages 217-23

Stewart V, Landick R, Yanofsky C

Abstract

Recent studies have suggested that expression of the tryptophanase (tna) operon of Escherichia coli is subject to transcription termination-antitermination control (V. Stewart and C. Yanofsky, J. Bacteriol. 164:731-740, 1985). In vivo studies have indicated that the transcribed leader region, tnaL, contains a site or sites of rho-dependent transcription termination (rho is the polypeptide product of the gene rho). We now report direct in vitro evidence that tnaL contains rho-dependent termination sites. In vivo termination appeared to occur at the rho-dependent termination sites identified in vitro. Transcription pausing analyses correlated sites of pausing in tnaL with sites of rho-dependent termination.

MeSH Terms
Base Sequence Escherichia coli/genetics Lyases/genetics Mutation Nucleic Acid Conformation Operon Promoter Regions, Genetic RNA, Bacterial/analysis Rho Factor/physiology Transcription Factors/physiology Transcription, Genetic Tryptophanase/genetics
Chemicals
RNA, Bacterial Rho Factor Transcription Factors Lyases Tryptophanase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Stewart V
Landick R
Yanofsky C
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38 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.166.1.217-223.1986
Published
1986-04-00
Pages
217-23
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC214579
Subset
IM
Grants
NIGMS NIH HHS · GM 08736 · United States
NIGMS NIH HHS · GM 09151 · United States
NIGMS NIH HHS · GM-09738 · United States
External Links
PubMed source DOI: 10.1128/jb.166.1.217-223.1986 PMC Full Text View journal details
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