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PMID: 6268608 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Nucleotide sequence of the structural gene for tryptophanase of Escherichia coli K-12.

Journal of bacteriology ·Vol. 147 ·No. 3 ·1981-09-00 ·Pages 787-96

Deeley MC, Yanofsky C

Abstract

The tryptophanase structural gene, tnaA, of Escherichia coli K-12 was cloned and sequenced. The size, amino acid composition, and sequence of the protein predicted from the nucleotide sequence agree with protein structure data previously acquired by others for the tryptophanase of E. coli B. Physiological data indicated that the region controlling expression of tnaA was present in the cloned segment. Sequence data suggested that a second structural gene of unknown function was located distal to tnaA and may be in the same operon. The pattern of codon usage in tnaA was intermediate between codon usage in four of the ribosomal protein structural genes and the structural genes for three of the tryptophan biosynthetic proteins.

MeSH Terms
Amino Acid Sequence Amino Acids/analysis Base Sequence Cloning, Molecular Codon DNA Restriction Enzymes Escherichia coli/genetics Genes Genes, Bacterial Lyases/genetics Operon Tryptophanase/analysis,genetics
Chemicals
Amino Acids Codon DNA Restriction Enzymes Lyases Tryptophanase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Deeley M C
Yanofsky C
References (31)
31 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.147.3.787-796.1981
Published
1981-09-00
Pages
787-96
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC216114
Subset
IM
Grants
NIGMS NIH HHS · GM09738 · United States
Databases
GENBANK
K00032
External Links
PubMed source DOI: 10.1128/jb.147.3.787-796.1981 PMC Full Text View journal details
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