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PMID: 2422314 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Localization of protective epitopes of the amino terminus of type 5 streptococcal M protein.

The Journal of experimental medicine ·Vol. 163 ·No. 5 ·1986-05-01 ·Pages 1191-202

Dale JB, Beachey EH

Abstract

We have used a set of overlapping chemically synthesized peptides representing the amino terminus of type 5 streptococcal M protein to localize protective, as opposed to nonprotective and tissue-crossreactive epitopes that might be appropriate for vaccine formulations. Rabbit antisera raised against SM5(1-35) reacted in high titer with pep M5 by ELISA and opsonized type 5 streptococci. None of the antisera crossreacted with human heart tissue or myosin. Antisera against SM5(26-35) reacted with SM5(1-35) and pep M5 but failed to opsonize type 5 streptococci. Particle-phase ELISA indicated that SM5(26-35) antibodies were directed against nonprotective determinants of pep M5 that were not exposed on the surface of viable organisms. Opsonization and ELISA inhibition assays showed that, of the SM5(1-35) antibodies that reacted with M5, all were inhibited by SM5(14-35), whereas none was inhibited by SM5(26-35), suggesting that the protective epitopes of SM5(1-35) resided between residues 14 and 26. This was confirmed by subsequent chemical synthesis of this region; SM5(14-26) totally inhibited SM5(1-35) antibodies that reacted with pep M5 in ELISA, and completely inhibited opsonization of type 5 streptococci by SM5(1-35) antibodies. SM5(14-26) evoked high titers of type-specific, opsonic antibodies against type 5 streptococci, confirming the protective immunogenicity of this 13-residue peptide of type 5 M protein.

MeSH Terms
Amino Acid Sequence Antibodies, Bacterial/biosynthesis Antibody Specificity Antigens, Bacterial/immunology Bacterial Outer Membrane Proteins Bacterial Proteins/immunology Bacterial Vaccines/immunology Carrier Proteins Cross Reactions Epitopes Peptides/chemical synthesis,immunology Streptococcus pyogenes/immunology
Chemicals
Antibodies, Bacterial Antigens, Bacterial Bacterial Outer Membrane Proteins Bacterial Proteins Bacterial Vaccines Carrier Proteins Epitopes Peptides streptococcal M protein
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Dale J B
Beachey E H
References (26)
26 references, click to expand
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.163.5.1191
Published
1986-05-01
Pages
1191-202
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2188111
Subset
IM
Grants
NIAID NIH HHS · AI-10085 · United States
NIAID NIH HHS · AI-13550 · United States
External Links
PubMed source DOI: 10.1084/jem.163.5.1191 PMC Full Text View journal details
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