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PMID: 2422658 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

T-lymphocyte differentiation and the extracellular matrix: identification of a thymocyte subset that attaches specifically to fibronectin.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 83 ·No. 8 ·1986-04-00 ·Pages 2647-51

Cardarelli PM, Pierschbacher MD

Abstract

A population of murine thymocytes adheres specifically to fibronectin but not to vitronectin, laminin, or collagen type I. The interaction of these thymocytes with fibronectin could be inhibited by the synthetic peptide Gly-Arg-Gly-Asp-Ser-Pro, which comprises the previously identified cell-attachment determinant of the molecule, suggesting that the cell attachment site on fibronectin is recognized by these cells. A similar peptide, in which the aspartate residue had been replaced with glutamate, had no effect on this adhesion. The fibronectin-adherent thymocytes were found to be cortisone-sensitive; to bind peanut agglutinin; to have a Thy-1.2+, Ia- surface phenotype; and to express H-2 antigen only weakly on their surface. In addition, approximately 80% of the fibronectin-adherent cells expressed L3T4 and 80% expressed Ly-1 on their surface, whereas greater than 95% were positive for Ly-2. The data suggest that these cells, which constitute 10% of all thymic lymphocytes, are cortical thymocytes. We propose that their adhesion to fibronectin may be important for their differentiation. The binding to fibronectin provides a means to selectively isolate these cells for study.

MeSH Terms
Animals Cell Adhesion Cell Differentiation Cell Line Collagen/metabolism Extracellular Matrix/cytology Fibronectins/metabolism Glycoproteins/metabolism Laminin/metabolism Mice T-Lymphocytes/cytology Vitronectin
Chemicals
Fibronectins Glycoproteins Laminin Vitronectin Collagen
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Cardarelli P M
Pierschbacher M D
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.83.8.2647
Published
1986-04-00
Pages
2647-51
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC323356
Subset
IM
Grants
NCI NIH HHS · CA30199 · United States
NCI NIH HHS · CA38352 · United States
External Links
PubMed source DOI: 10.1073/pnas.83.8.2647 PMC Full Text View journal details
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