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PMID: 2423324 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Alzheimer's paired helical filaments share epitopes with neurofilament side arms.

The EMBO journal ·Vol. 5 ·No. 2 ·1986-02-00 ·Pages 269-76

Miller CC, Brion JP, Calvert R, Chin TK, Eagles PA, Downes MJ, Flament-Durand J, Haugh M, Kahn J, Probst A

Abstract

A panel of monoclonal antibodies to neurofilaments have been investigated with regard to the location of their respective epitopes on neurofilament polypeptides and their ability to label the neurofibrillary tangles and paired helical filaments (PHF) which are characteristic of Alzheimer's disease. All of the neurofilament monoclonal antibodies that label tangles and PHF are directed against epitopes in the side arm domains of the two larger neurofilament polypeptides, NF-H and NF-M, and do not recognise the alpha-helical rod domains of these proteins. Immuno-electron microscopy demonstrates that the neurofilament antibodies label the constituent PHF per se and do not simply stain neurofilaments that might be admixed with PHF. These neurofilament epitopes are differentially retained by PHF, following isolation. Thus, antibody labelling of PHF is not simply due to the presence of normal neurofilament polypeptides. We propose that in tangle-bearing neurons, neurofilaments are degraded by proteases and that it is fragments of the side arms which contribute to the composition of PHF.

MeSH Terms
Alzheimer Disease/pathology Animals Antibodies, Monoclonal Brain/ultrastructure Cattle Cerebral Cortex/ultrastructure Cytoskeleton/ultrastructure Electrophoresis, Polyacrylamide Gel Epitopes/analysis Humans Intermediate Filaments/ultrastructure Microscopy, Electron Neurofibrils/ultrastructure Organ Specificity Rats Spinal Cord/ultrastructure
Chemicals
Antibodies, Monoclonal Epitopes
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Miller C C
Brion J P
Calvert R
Chin T K
Eagles P A
Downes M J
Flament-Durand J
Haugh M
Kahn J
Probst A
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1986-02-00
Pages
269-76
Language
English
Region
England
NLM ID
8208664
PMCID
PMC1166729
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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