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PMID: 2423848 Published · ppublish English Journal Article

Cloning part of the region encoding biosynthetic enzymes for surface antigen (O-antigen) of Salmonella typhimurium.

Molecular & general genetics : MGG ·Vol. 203 ·No. 1 ·1986-04-00 ·Pages 172-6

Brahmbhatt HN, Quigley NB, Reeves PR

Abstract

The rfb gene cluster of Salmonella typhimurium encodes the enzymes required for the biosynthesis of the O-Antigen. A part of it has been cloned in plasmid vectors pBR322 and pUC9 using an adjacent, previously cloned, part of the his operon (Barnes 1981) as a molecular probe for the first clone. A detailed restriction enzyme map of 7.57 kb of rfb DNA is presented and the approximate locations of two of the genes, rfbK and rfbM have been defined.

MeSH Terms
Antigens, Bacterial/genetics Antigens, Surface/genetics Cloning, Molecular DNA Restriction Enzymes Genes Genes, Bacterial Genetic Vectors Lipopolysaccharides/genetics O Antigens Plasmids Salmonella typhimurium/genetics,immunology
Chemicals
Antigens, Bacterial Antigens, Surface Lipopolysaccharides O Antigens DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Brahmbhatt H N
Quigley N B
Reeves P R
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00330399
Published
1986-04-00
Pages
172-6
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00330399
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