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PMID: 2424019 Published · ppublish English Journal Article

Transforming growth factor type beta: rapid induction of fibrosis and angiogenesis in vivo and stimulation of collagen formation in vitro.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 83 ·No. 12 ·1986-06-00 ·Pages 4167-71

Roberts AB, Sporn MB, Assoian RK, Smith JM, Roche NS, Wakefield LM, Heine UI, Liotta LA, Falanga V, Kehrl JH

Abstract

Transforming growth factor type beta (TGF-beta), when injected subcutaneously in newborn mice, causes formation of granulation tissue (induction of angiogenesis and activation of fibroblasts to produce collagen) at the site of injection. These effects occur within 2-3 days at dose levels than 1 microgram. Parallel in vitro studies show that TGF-beta causes marked increase of either proline or leucine incorporation into collagen in either an NRK rat fibroblast cell line or early passage human dermal fibroblasts. Epidermal growth factor (EGF) and platelet-derived growth factor (PDGF) do not cause these same in vivo and in vitro effects; in both rat and human fibroblast cultures, EGF antagonizes the effects of TGF-beta on collagen formation. We have obtained further data to support a role for TGF-beta as an intrinsic mediator of collagen formation: conditioned media obtained from activated human tonsillar T lymphocytes contain greatly elevated levels of TGF-beta compared to media obtained from unactivated lymphocytes. These activated media markedly stimulate proline incorporation into collagen in NRK cells; this effect is blocked by a specific antibody to TGF-beta. The data are all compatible with the hypothesis that TGF-beta is an important mediator of tissue repair.

MeSH Terms
Amino Acids/metabolism Animals Biological Transport/drug effects Collagen/biosynthesis Epidermal Growth Factor/pharmacology Granuloma/chemically induced Growth Substances/pharmacology Humans Lymphocytes/physiology Lymphokines/pharmacology Mice Neovascularization, Pathologic Peptides/pharmacology Rats Transforming Growth Factors
Chemicals
Amino Acids Growth Substances Lymphokines Peptides Epidermal Growth Factor Transforming Growth Factors Collagen
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Roberts A B
Sporn M B
Assoian R K
Smith J M
Roche N S
Wakefield L M
Heine U I
Liotta L A
Falanga V
Kehrl J H
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.83.12.4167
Published
1986-06-00
Pages
4167-71
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC323692
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.83.12.4167 PMC Full Text View journal details
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