Six grasses, Hordeum sativum, Dactylis glomerata, Festuca arundinacea, F. pratensis, F. rubra and Lolium multiflorum were subjected to chloroplast DNA analysis based on restriction endonuclease digestion fragments and end labeling with (35)S nucleotides. This method is compared with others in general use. The results indicate that Lolium multiflorum is closely affiliated with Festuca pratensis and F. arundinacea; in fact much closer than F. rubra is to any of them.
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