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PMID: 2424833 Published · ppublish English Journal Article

Functional definition of HLA-DR and -DQ epitopes specific for DR2-short, DwFJO haplotype.

Immunogenetics ·Vol. 23 ·No. 6 ·1986-00-00 ·Pages 385-92

Zeliszewski D, Sterkers G, Freidel C, Gebuhrer L, Betuel H, Levy JP

Abstract

T-lymphocyte clones specific for the influenza A/Texas virus were obtained by limiting dilution of activated T cells from an HLA A2/3,B7/39,Cw -/-,DR2-short/2 short,DQw1,DwFJO/FJO donor. Among the proliferating clones studied, and irrespective of their antigenic specificities, most of them were restricted by epitope(s) on HLA-DR molecules present only on DR2-short/DwFJO cells but not on DR2-negative or DR2-long positive (Dw2,Dw12,Dw-) cells. Two clones were restricted by epitopes borne by DQ products. Here again, these epitopes were present on DR2-short/DwFJO but not on DR2-long,DQw1 (Dw2,Dw12) cells, indicating that the DQw1 molecules of DR2-long and DR2-short haplotypes are different. Taken together, these results indicate that the DR2-short,DwFJO haplotype is characterized by both HLA-DR- and DQ-specific molecules. Finally, one clone was restricted by an epitope shared by DR products from DR2 short/DwFJO, DRw11, and DRw13 haplotypes. This latter functional determinant has never been described until now.

MeSH Terms
Antibodies, Monoclonal/immunology Clone Cells/immunology Epitopes/immunology HLA-DQ Antigens HLA-DR Antigens Histocompatibility Antigens Class II/immunology Humans Influenza A virus/immunology Phenotype T-Lymphocytes, Cytotoxic/immunology
Chemicals
Antibodies, Monoclonal Epitopes HLA-DQ Antigens HLA-DR Antigens Histocompatibility Antigens Class II
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Zeliszewski D
Sterkers G
Freidel C
Gebuhrer L
Betuel H
Levy J P
References (23)
23 references, click to expand
  1. Characterization and expression of the HLA-DC antigens defined by anti-Leu 10.
    Hum Immunol. 1984 Aug;10(4):221-35 PMID: 6332101
  2. Two populations of Ia-like molecules on a human B cell line.
    J Immunol. 1980 Jul;125(1):293-9 PMID: 6966655
  3. Definition of a new DR2 short associated HLA-D specificity with the Ninth Histocompatibility Workshop reagents.
    Tissue Antigens. 1984 Sep;24(3):184-6 PMID: 6595838
  4. Five HLA-D clusters associated with HLA-DR4.
    Hum Immunol. 1982 Jun;4(3):249-58 PMID: 6181034
  5. Demonstration at the single-cell level of the existence of distinct clusters of epitopes in two predefined human Ia molecular subsets.
    Eur J Immunol. 1982 Feb;12(2):166-9 PMID: 6176457
  6. Characterization of human T-lymphocyte clones (TLCs) specific for HLA-region gene products.
    Immunogenetics. 1982;16(2):117-33 PMID: 6183195
  7. Antigen-specific human T lymphocyte clones: induction, antigen specificity, and MHC restriction of influenza virus-immune clones.
    J Immunol. 1982 Jan;128(1):233-8 PMID: 6172480
  8. Functional subdivision of HLA-DRw8 with influenza-specific cloned cell lines.
    Immunogenetics. 1985;22(4):407-12 PMID: 3876990
  9. Multiple Ia-like molecules characterize HLA-DR2-associated haplotypes which differ in HLA-D.
    Hum Immunol. 1984 Jul;10(3):143-51 PMID: 6430842
  10. A possible new HLA-DR allele.
    Hum Immunol. 1983 Nov;8(3):227-37 PMID: 6196341
  11. A study of HLA-DR2 associated HLA-Dw/LD specificities.
    Hum Immunol. 1984 Oct;11(2):105-15 PMID: 6208174
  12. Interdependent membrane mobility of human MHC coded antigens detected by monoclonal antibodies to various epitopes on class I and II molecules.
    Tissue Antigens. 1985 Jul;26(1):51-9 PMID: 2412307
  13. T-cell clones with similar antigen specificity may be restricted by DR, MT(DC), or SB class II HLA molecules.
    Immunogenetics. 1984;19(5):455-60 PMID: 6427104
  14. A novel HLA-D/DR-like antigen specific for human B lymphoid cells. Biochemical evidence for similarity to but nonidentity with known HLA-D/DR antigens.
    J Exp Med. 1983 Dec 1;158(6):1924-37 PMID: 6606012
  15. Fine specificity analysis of human influenza-specific cloned cell lines.
    Cell Immunol. 1985 Sep;94(2):394-405 PMID: 2411429
  16. Production of monoclonal antibodies to group A erythrocytes, HLA and other human cell surface antigens-new tools for genetic analysis.
    Cell. 1978 May;14(1):9-20 PMID: 667938
  17. Splits of the HLA DR2 antigen.
    Tissue Antigens. 1983 Jul;22(1):29-31 PMID: 6193604
  18. HLA-D clusters associated with DR2 and the definition of HLA-D"AZH": a new DR2 related HLA-D specificity in Israel.
    Tissue Antigens. 1984 Jul;24(1):1-9 PMID: 6207623
  19. Split of HLA-DRw2 into subtypic specificities closely correlated to two HLA-D products.
    Immunogenetics. 1978 Dec;7(1):57-61 PMID: 21302057
  20. Monoclonal antibodies to HLA--DRw determinants.
    Tissue Antigens. 1980 Jul;16(1):30-48 PMID: 6162228
  21. Influenza A hemagglutinin-specific T cell clones strictly restricted by HLA-DR1 or HLA-DR7 molecules.
    Eur J Immunol. 1984 Feb;14(2):125-32 PMID: 6199211
  22. Human Ia molecules carrying DC1 determinants differ in both alpha- and beta-subunits from Ia molecules carrying DR determinants.
    Nature. 1981 Jul 23;292(5821):357-60 PMID: 6166865
  23. Structural polymorphism of the DC1 light chains from DR1-, DR2-, and DRw6-positive cell lines.
    Immunogenetics. 1983;18(6):625-37 PMID: 6581120
Article Info
Journal
Immunogenetics
Abbr.
Immunogenetics
ISSN
0093-7711
DOI
10.1007/BF00372671
Published
1986-00-00
Pages
385-92
Language
English
Region
United States
NLM ID
0420404
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00372671 View journal details
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