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PMID: 6183195 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Characterization of human T-lymphocyte clones (TLCs) specific for HLA-region gene products.

Immunogenetics ·Vol. 16 ·No. 2 ·1982-00-00 ·Pages 117-33

Eckels DD, Hartzman RJ

Abstract

Clones of lymphocytes, primed in vitro to HLA-DR1;Dw1, were tested for allospecific proliferation on a panel of thirty-one HLA-phenotyped stimulating cells. No clone was restimulated exclusively by cells sharing the DR1;Dw1 priming antigens and most clones were restimulated by subsets of cells bearing DR1;Dw1. Generally, positive responses were at least 20-fold higher than autologous negative controls. Peak proliferative responses occurred around 72 h and varied, depending on the stimulating cell as well as the responding clone. Selected clones were induced to proliferate only by cells incapable of forming rosettes with sheep erythrocytes. Specific proliferation by TLCs was blocked by monoclonal DR-specific antibodies, but not by monoclonal anti-Thy 1.2. Genetic studies demonstrated that TLCs detected some cell-surface determinants that are encoded by genes in linkage disequilibrium with HLA and others that may not be linked to the human major histocompatibility complex.

MeSH Terms
Antibodies, Monoclonal/immunology Clone Cells/immunology Epitopes/immunology Genes, MHC Class II HLA Antigens/genetics,immunology HLA-DR1 Antigen Histocompatibility Antigens Class II/immunology Humans Lymphocyte Activation Lymphocyte Culture Test, Mixed Phenotype T-Lymphocytes/immunology
Chemicals
Antibodies, Monoclonal Epitopes HLA Antigens HLA-DR1 Antigen Histocompatibility Antigens Class II
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Eckels D D
Hartzman R J
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34 references, click to expand
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Article Info
Journal
Immunogenetics
Abbr.
Immunogenetics
ISSN
0093-7711
DOI
10.1007/BF00364399
Published
1982-00-00
Pages
117-33
Language
English
Region
United States
NLM ID
0420404
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00364399 View journal details
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