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PMID: 24254124 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Comparing the calcium binding abilities of two soybean calmodulins: towards understanding the divergent nature of plant calmodulins.

The Plant cell ·Vol. 25 ·No. 11 ·2013-11-00 ·Pages 4512-24

Gifford JL, Jamshidiha M, Mo J, Ishida H, Vogel HJ

Abstract

The discovery that plants contain multiple calmodulin (CaM) isoforms of variable sequence identity to animal CaM suggested an additional level of sophistication in the intracellular role of calcium regulation in plants. Past research has focused on the ability of conserved or divergent plant CaM isoforms to activate both mammalian and plant protein targets. At present, however, not much is known about how these isoforms respond to the signal of an increased cytosolic calcium concentration. Here, using isothermal titration calorimetry and NMR spectroscopy, we investigated the calcium binding properties of a conserved (CaM1) and a divergent (CaM4) CaM isoform from soybean (Glycine max). Both isoforms bind calcium with a semisequential pathway that favors the calcium binding EF-hands of the C-terminal lobe over those of the N-terminal lobe. From the measured dissociation constants, CaM4 binds calcium with a threefold greater affinity than CaM1 (K(d,Ca,mean) of 5.0 versus 14.9 μM) but has a significantly reduced selectivity against the chemically similar magnesium cation that binds preferentially to EF-hand I of both isoforms. The implications of a potential magnesium/calcium competition on the activation of CaM1 and CaM4 are discussed in context with their ability to respond to stimulus-specific calcium signatures and their known physiological roles.

MeSH Terms
Binding Sites Binding, Competitive Calcium/metabolism Calmodulin/chemistry,metabolism Entropy Magnesium/metabolism Magnetic Resonance Spectroscopy Plant Proteins/chemistry,metabolism Protein Conformation Protein Interaction Domains and Motifs Protein Isoforms Soybeans/chemistry,metabolism Spectrometry, Fluorescence
Chemicals
Calmodulin Plant Proteins Protein Isoforms Magnesium Calcium
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Gifford Jessica L
Biochemistry Research Group, Department of Biological Sciences, University of Calgary T2N 1N4, Calgary, Alberta, Canada.
Jamshidiha Mostafa
Mo Jeffrey
Ishida Hiroaki
Vogel Hans J
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1532-298X
DOI
10.1105/tpc.113.113183
Published
2013-11-00
Epub
2013-00-19
Pages
4512-24
Language
English
Region
England
NLM ID
9208688
PMCID
PMC3875733
Subset
IM
External Links
PubMed source DOI: 10.1105/tpc.113.113183 PMC Full Text View journal details
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