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PMID: 2426583 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Regulation of cellular phenotype and expression of polyomavirus middle T antigen in rat fibroblasts.

Molecular and cellular biology ·Vol. 5 ·No. 9 ·1985-09-00 ·Pages 2476-86

Raptis L, Lamfrom H, Benjamin TL

Abstract

Polyoma middle T antigen (mT) was expressed in rat F-111 cells under control of the dexamethasone-regulatable mouse mammary tumor virus promoter. Graded phenotypic responses to levels of mT induction by the hormone were seen, with morphological transformation, focus formation, and anchorage-independent growth requiring increasing levels of mT expression. The ability of different clones to form tumors reflected their maximum level of induction of mT-associated kinase and their ability to grow in soft agar. Expression of transformation parameters and tumorigenicity correlates with the level of mT phosphorylated by pp60c-src in immune complexes and not with the total amount of mT determined by metabolic labeling. We suggest that cellular factors regulate mT activity by forming a kinase-active fraction of mT molecules that controls the transformed state.

MeSH Terms
Animals Antigens, Polyomavirus Transforming Antigens, Viral, Tumor/genetics,physiology Cell Adhesion Cell Line Cell Transformation, Viral Dexamethasone/pharmacology Fibroblasts/metabolism Gene Expression Regulation/drug effects Genes, Synthetic Male Mammary Tumor Virus, Mouse/genetics Neoplasm Transplantation Oncogene Proteins, Viral/genetics,physiology Phenotype Polyomavirus/genetics,immunology,physiology Promoter Regions, Genetic Protein Kinases/genetics,physiology Proto-Oncogene Proteins/metabolism Proto-Oncogene Proteins pp60(c-src) Rats Recombinant Proteins/genetics,physiology
Chemicals
Antigens, Polyomavirus Transforming Antigens, Viral, Tumor Oncogene Proteins, Viral Proto-Oncogene Proteins Recombinant Proteins Dexamethasone Protein Kinases Proto-Oncogene Proteins pp60(c-src)
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Raptis L
Lamfrom H
Benjamin T L
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37 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.5.9.2476-2486.1985
Published
1985-09-00
Pages
2476-86
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC366975
Subset
IM
Grants
NCI NIH HHS · CA19567 · United States
External Links
PubMed source DOI: 10.1128/mcb.5.9.2476-2486.1985 PMC Full Text View journal details
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