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PMID: 9279398 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Expression of the mouse dihydrofolate reductase complementary deoxyribonucleic acid in simian virus 40 vectors.

Molecular and cellular biology ·Vol. 1 ·No. 9 ·1981-09-00 ·Pages 854-64

Subramani S, Mulligan R, Berg P

Abstract

A mouse complementary deoxyribonucleic acid segment coding for the enzyme dihydrofolate reductase has been cloned in two general classes of vectors containing simian virus 40 deoxyribonucleic acid: (i) those that can be propagated as virions in permissive cells and (ii) those that can be introduced into and maintained stably in various mammalian cells. Both types of vectors express the mouse dihydrofolate reductase by using signals supplied by simian virus 40 deoxyribonucleic acid sequences. Moreover, plasmid vectors carrying the complementary deoxyribonucleic acid segment can complement Chinese hamster ovary cells lacking dihydrofolate reductase.

MeSH Terms
Animals CHO Cells Cloning, Molecular Cricetinae DNA, Complementary/metabolism Genetic Vectors Haplorhini Mice Phenotype RNA Splicing RNA, Messenger/metabolism Simian virus 40/genetics Tetrahydrofolate Dehydrogenase/genetics
Chemicals
DNA, Complementary RNA, Messenger Tetrahydrofolate Dehydrogenase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Subramani S
Department of Biochemistry, Stanford University School of Medicine, California 94305, USA.
Mulligan R
Berg P
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.1.9.854-864.1981
Published
1981-09-00
Pages
854-64
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC369369
Subset
IM
Grants
NCI NIH HHS · CA 15513-07 · United States
NIGMS NIH HHS · GM-13235 · United States
External Links
PubMed source DOI: 10.1128/mcb.1.9.854-864.1981 PMC Full Text View journal details
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