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PMID: 2427747 Published · ppublish English Journal Article

Murine leukemia virus pol gene products: analysis with antisera generated against reverse transcriptase and endonuclease fusion proteins expressed in Escherichia coli.

Journal of virology ·Vol. 60 ·No. 1 ·1986-10-00 ·Pages 267-74

Hu SC, Court DL, Zweig M, Levin JG

Abstract

The organization of the murine leukemia virus (MuLV) pol gene was investigated by expressing molecular clones containing AKR MuLV reverse transcriptase or endonuclease or both gene segments in Escherichia coli and generating specific antisera against the expressed bacterial proteins. Reaction of these antisera with detergent-disrupted virus precipitated an 80-kilodalton (kDa) protein, the MuLV reverse transcriptase, and a 46-kDa protein which we believe is the viral endonuclease. A third (50-kDa) protein, related to reverse transcriptase, was also precipitated. Bacterial extracts of clones expressing reverse transcriptase and endonuclease sequences competed with the viral 80- and 46-kDa proteins, respectively. These results demonstrate that the antisera are specific for viral reverse transcriptase and endonuclease. Immunoprecipitation of AKR MuLV with antisera prepared against a bacterial protein containing only endonuclease sequences led to the observation that reverse transcriptase and endonuclease can be associated as a complex involving a disulfide bond(s).

MeSH Terms
AKR murine leukemia virus/genetics,immunology Endonucleases/genetics,immunology Escherichia coli/genetics Genes Genes, Viral Leukemia Virus, Murine/genetics Protein Conformation RNA-Directed DNA Polymerase/genetics,immunology Recombinant Proteins/genetics,immunology Retroviridae Proteins/genetics,immunology
Chemicals
Recombinant Proteins Retroviridae Proteins RNA-Directed DNA Polymerase Endonucleases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Hu S C
Court D L
Zweig M
Levin J G
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.60.1.267-274.1986
Published
1986-10-00
Pages
267-74
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC253925
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.60.1.267-274.1986 PMC Full Text View journal details
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