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PMID: 2429314 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Cell type-specific negative regulatory element in the control region of the rat alpha-fetoprotein gene.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 83 ·No. 20 ·1986-10-00 ·Pages 7653-7

Muglia L, Rothman-Denes LB

Abstract

Albumin and alpha-fetoprotein are evolutionarily related genes that show differential and complex patterns of regulation during development. We investigated the role of the sequences flanking the transcription initiation site of the rat alpha-fetoprotein gene in transient transfection assays of hepatic and nonhepatic cell lines. Chimeric flanking regions and deletion analysis have defined the following three functionally different regions: a cell type-specific enhancer(s), encompassing 3 kilobases, located between -7 kilobase pairs and -4 kilobase pairs; a cell type-specific promoter, inactive in the absence of an enhancer and comprising at most the 180 base pairs upstream from the site of transcription initiation; and a 550-base-pair region, located between the promoter and the enhancer (at -3.5 kilobases), down-regulates transcription initiation in a cell type-specific manner and is also capable of repressing heterologous promoters. The implications of these findings with respect to the complex pattern of AFP regulation are discussed.

MeSH Terms
Albumins/genetics Enhancer Elements, Genetic Gene Expression Regulation Genes, Regulator Promoter Regions, Genetic Transcription, Genetic alpha-Fetoproteins/genetics
Chemicals
Albumins alpha-Fetoproteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Muglia L
Rothman-Denes L B
References (36)
36 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.83.20.7653
Published
1986-10-00
Pages
7653-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC386779
Subset
IM
Grants
NIAID NIH HHS · R01 AI 12575 · United States
External Links
PubMed source DOI: 10.1073/pnas.83.20.7653 PMC Full Text View journal details
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