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PMID: 2430046 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Class II histocompatibility antigen expression in human melanocytes transformed by Harvey murine sarcoma virus (Ha-MSV) and Kirsten MSV retroviruses.

The Journal of experimental medicine ·Vol. 164 ·No. 5 ·1986-11-01 ·Pages 1710-22

Albino AP, Houghton AN, Eisinger M, Lee JS, Kantor RR, Oliff AI, Old LJ

Abstract

Human melanocytes infected with Ki-MSV or Ha-MSV, but not amphotropic MuLV, undergo a series of transformation-related changes that are characteristic of malignant melanoma. These are (a) expression of Ia antigens, in particular DP, DQ, and DR class II histocompatibility gene products, (b) a transformed morphology and ability to grow in soft agar, and (c) a 5-10-fold increase in the cell surface expression of GD3 ganglioside. However, other characteristics of melanoma, such as independence from specific growth factors and loss of adenosine deaminase binding protein were not observed. We conclude that viral ras oncogenes initiate early transformation events in melanocytes, and that Ia antigen expression is a transformation marker in this system.

MeSH Terms
Cell Division Cell Transformation, Neoplastic Cell Transformation, Viral Cells, Cultured Harvey murine sarcoma virus Histocompatibility Antigens Class II/analysis,genetics Humans Interferon-gamma/biosynthesis Kirsten murine sarcoma virus Melanocytes/immunology,pathology Melanoma/immunology Oncogenes RNA/analysis Tetradecanoylphorbol Acetate/pharmacology
Chemicals
Histocompatibility Antigens Class II RNA Interferon-gamma Tetradecanoylphorbol Acetate
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Albino A P
Houghton A N
Eisinger M
Lee J S
Kantor R R
Oliff A I
Old L J
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.164.5.1710
Published
1986-11-01
Pages
1710-22
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2188468
Subset
IM
Grants
NCI NIH HHS · CA-32152 · United States
NCI NIH HHS · CA-37907 · United States
External Links
PubMed source DOI: 10.1084/jem.164.5.1710 PMC Full Text View journal details
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