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PMID: 6270351 Published · ppublish English Comparative Study Journal Article

Genomes of murine leukemia viruses isolated from wild mice.

Journal of virology ·Vol. 39 ·No. 3 ·1981-09-00 ·Pages 777-91

Chattopadhyay SK, Oliff AI, Linemeyer DL, Lander MR, Lowy DR

Abstract

The genomes of murine leukemia viruses (MuLV) isolated from wild mice have been studied. Detailed restriction endonuclease maps of the 8.8-kilobase (kb) unintegrated linear viral DNAs were derived for five ecotropic and five amphotropic MuLV's from California field mice, for Friend MuLV, and for one ecotropic and one xenotropic MuLV from Mus musculus castaneus. In general, the California MuLV's were similar in their leftward 6 kb (corresponding to the leftward long terminal repeat [LTR], gag, and pol) and rightward 1 kb (7.8 to 8.8 kb, corresponding to p15E and the rightward LTR). For the region spanning 6.0 to 7.7 kb (which includes the sequences that encode gp70) the amphotropic MuLV's shared few enzyme sites with the ecotropic MuLV's, although the California ecotropic MuLV's were highly related to each other in this region, as were the amphotropic MuLV's. Cross-hybridization studies between amphotropic and California ecotropic MuLV DNAs indicated that they were not homologous in the region 6.3 to 7.6 kb; the California ecotropic viral DNAs cross-hybridized in this region to AKR ecotropic MuLV. When the California viral DNAs were compared with AKR ecotropic viral DNA, many differences in enzyme sites were noted throughout the genome. The U3 regions of the wild mouse LTRs showed partial homology to this region in AKR MuLV. The LTR of Moloney MuLV was highly related to that of the California MuLV's, whereas the LTR of Friend MuLV appeared to be a recombinant between the two types of LTRs. The M. musculus castaneus isolates were most closely related to ecotropic and xenotropic MuLV's isolated from inbred mice. One amphotropic MuLV DNA was cloned from supercoiled viral DNA at its unique EcoRI site in pBR322. Viral DNAs with one and two LTRs were isolated. After digestion with EcoRI, DNAs of both types were infectious. It is concluded that ecotropic and amphotropic MuLV's differ primarily in the region which encodes gp70.

MeSH Terms
Animals Animals, Wild/microbiology Base Sequence Cloning, Molecular DNA Restriction Enzymes DNA, Viral Friend murine leukemia virus/genetics Genes, Viral Leukemia Virus, Murine/genetics,isolation & purification Mice/microbiology Muridae/microbiology Nucleic Acid Hybridization
Chemicals
DNA, Viral DNA Restriction Enzymes
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Chattopadhyay S K
Oliff A I
Linemeyer D L
Lander M R
Lowy D R
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46 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.39.3.777-791.1981
Published
1981-09-00
Pages
777-91
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC171310
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.39.3.777-791.1981 PMC Full Text View journal details
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