Home LiteratureArticle Details
PMID: 2431291 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Alternative RNA splicing generates transcripts encoding a thorax-specific isoform of Drosophila melanogaster myosin heavy chain.

Molecular and cellular biology ·Vol. 6 ·No. 7 ·1986-07-00 ·Pages 2511-9

Bernstein SI, Hansen CJ, Becker KD, Wassenberg DR, Roche ES, Donady JJ, Emerson CP

Abstract

Genomic and cDNA sequencing studies show that transcripts from the muscle myosin heavy-chain (MHC) gene of Drosophila melanogaster are alternatively spliced, producing RNAs that encode at least two MHC isoforms with different C termini. Transcripts encoding an MHC isoform with 27 unique C-terminal amino acids accumulate during both larval and adult muscle differentiation. Transcripts for the second isoform encode one unique C-terminal amino acid and accumulate almost exclusively in pupal and adult thoracic segments, the location of the indirect flight muscles. The 3' splice acceptor site preceding the thorax-specific exon is unusually purine rich and thus may serve as a thorax-specific splicing signal. We suggest that the alternative C termini of these two MHC isoforms control myofilament assembly and may play a role in generating the distinctive myofilament organizations of flight muscle and other muscle types.

MeSH Terms
Amino Acid Sequence Animals Base Sequence DNA/analysis Drosophila melanogaster/enzymology Endonucleases/metabolism Gene Expression Regulation Isoenzymes/genetics Myosins/genetics RNA/metabolism RNA Splicing Single-Strand Specific DNA and RNA Endonucleases Transcription, Genetic
Chemicals
Isoenzymes RNA DNA Endonucleases Single-Strand Specific DNA and RNA Endonucleases Myosins
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Bernstein S I
Hansen C J
Becker K D
Wassenberg D R
Roche E S
Donady J J
Emerson C P
References (59)
59 references, click to expand
  1. The duplicated human alpha-globin genes: their relative expression as measured by RNA analysis.
    Cell. 1981 May;24(2):345-51 PMID: 7237552
  2. Differential processing of RNA transcribed from the single-copy Drosophila myosin heavy chain gene produces four mRNAs that encode two polypeptides.
    Proc Natl Acad Sci U S A. 1986 Apr;83(7):2128-32 PMID: 3083414
  3. Isolation of multiple genomic sequences coding for chicken myosin heavy chain protein.
    J Biol Chem. 1982 Jan 10;257(1):549-56 PMID: 6273442
  4. The free heavy chain of vertebrate skeletal myosin subfragment 1 shows full enzymatic activity.
    J Biol Chem. 1982 Jan 25;257(2):1102-5 PMID: 6119311
  5. Isolation of Drosophila flightless mutants which affect myofibrillar proteins of indirect flight muscle.
    Mol Gen Genet. 1981;183(3):409-17 PMID: 6801424
  6. A catalogue of splice junction sequences.
    Nucleic Acids Res. 1982 Jan 22;10(2):459-72 PMID: 7063411
  7. Localization of the three phosphorylation sites on each heavy chain of Acanthamoeba myosin II to a segment at the end of the tail.
    J Biol Chem. 1982 Apr 25;257(8):4529-34 PMID: 7200093
  8. cDNA clone analysis of six co-regulated mRNAs encoding skeletal muscle contractile proteins.
    Proc Natl Acad Sci U S A. 1982 Mar;79(5):1553-7 PMID: 6951196
  9. Molecular characterization of two myosin heavy chain genes expressed in the adult heart.
    Nature. 1982 Jun 24;297(5868):659-64 PMID: 7045682
  10. Periodic charge distributions in the myosin rod amino acid sequence match cross-bridge spacings in muscle.
    Nature. 1982 Sep 16;299(5880):226-31 PMID: 7202124
  11. Transposition of cloned P elements into Drosophila germ line chromosomes.
    Science. 1982 Oct 22;218(4570):341-7 PMID: 6289435
  12. Genetic transformation of Drosophila with transposable element vectors.
    Science. 1982 Oct 22;218(4570):348-53 PMID: 6289436
  13. Characterization of sarcomeric myosin heavy chain genes.
    J Biol Chem. 1983 Jan 10;258(1):670-8 PMID: 6294119
  14. Drosophila has one myosin heavy-chain gene with three developmentally regulated transcripts.
    Cell. 1983 Jan;32(1):23-34 PMID: 6402306
  15. Cloned mRNA sequences for two types of embryonic myosin heavy chains from chick skeletal muscle. I. DNA and derived amino acid sequence of light meromyosin.
    J Biol Chem. 1983 Apr 25;258(8):5196-205 PMID: 6833296
  16. Cloned mRNA sequences for two types of embryonic myosin heavy chains from chick skeletal muscle. II. Expression during development using S1 nuclease mapping.
    J Biol Chem. 1983 Apr 25;258(8):5206-14 PMID: 6300120
  17. Drosophila muscle myosin heavy chain encoded by a single gene in a cluster of muscle mutations.
    Nature. 1983 Mar 31-Apr 6;302(5907):393-7 PMID: 6403869
  18. Specific transcription and RNA splicing defects in five cloned beta-thalassaemia genes.
    Nature. 1983 Apr 14;302(5909):591-6 PMID: 6188062
  19. Isolation and characterization of human myosin heavy chain genes.
    Proc Natl Acad Sci U S A. 1983 Jun;80(12):3716-20 PMID: 6304733
  20. Protein structural domains in the Caenorhabditis elegans unc-54 myosin heavy chain gene are not separated by introns.
    Proc Natl Acad Sci U S A. 1983 Jul;80(14):4253-7 PMID: 6576334
  21. Monoclonal antibodies localize changes on myosin heavy chain isozymes during avian myogenesis.
    Cell. 1983 Aug;34(1):295-306 PMID: 6192935
  22. Differential localization of two myosins within nematode thick filaments.
    Cell. 1983 Sep;34(2):477-90 PMID: 6352051
  23. Sequential accumulation of mRNAs encoding different myosin heavy chain isoforms during skeletal muscle development in vivo detected with a recombinant plasmid identified as coding for an adult fast myosin heavy chain from mouse skeletal muscle.
    J Biol Chem. 1983 Nov 25;258(22):13867-74 PMID: 6196357
  24. Actin-myosin interaction: the role of myosin in determining the actin pattern in self-assembled 'hybrid' contractile units.
    Tissue Cell. 1983;15(6):955-63 PMID: 6665785
  25. Alternative transcription and two modes of splicing results in two myosin light chains from one gene.
    Nature. 1984 Mar 22-28;308(5957):333-8 PMID: 6709041
  26. The messenger RNA for alcohol dehydrogenase in Drosophila melanogaster differs in its 5' end in different developmental stages.
    Cell. 1983 May;33(1):125-33 PMID: 6432335
  27. Excision of an intact intron as a novel lariat structure during pre-mRNA splicing in vitro.
    Cell. 1984 Aug;38(1):317-31 PMID: 6088074
  28. Direct localization of monoclonal antibody-binding sites on Acanthamoeba myosin-II and inhibition of filament formation by antibodies that bind to specific sites on the myosin-II tail.
    J Cell Biol. 1984 Sep;99(3):1015-23 PMID: 6206074
  29. Alternative RNA processing: determining neuronal phenotype.
    Science. 1984 Sep 21;225(4668):1315-20 PMID: 6089345
  30. Myosin.
    Annu Rev Biochem. 1984;53:35-73 PMID: 6383197
  31. A single locus in the mouse encodes both myosin light chains 1 and 3, a second locus corresponds to a related pseudogene.
    Cell. 1984 Nov;39(1):129-40 PMID: 6091905
  32. Tissue-specific generation of two preprotachykinin mRNAs from one gene by alternative RNA splicing.
    Nature. 1984 Dec 20-1985 Jan 2;312(5996):729-34 PMID: 6083453
  33. ATPase activity of myosin correlated with speed of muscle shortening.
    J Gen Physiol. 1967 Jul;50(6):Suppl:197-218 PMID: 4227924
  34. Actin-myosin interaction. Self-assembly into a bipolar "contractile unit".
    J Mol Biol. 1977 Apr;111(2):159-71 PMID: 323501
  35. Isolation and partial characterization of Drosophila myoblasts from primary cultures of embryonic cells.
    J Cell Biol. 1978 Sep;78(3):856-65 PMID: 100502
  36. Myosin isoenzyme redistribution in chronic heart overload.
    Nature. 1979 Nov 1;282(5734):105-7 PMID: 91973
  37. The actin genes of Drosophila: a dispersed multigene family.
    Cell. 1980 Feb;19(2):365-78 PMID: 6244106
  38. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  39. Two mRNAs with different 3' ends encode membrane-bound and secreted forms of immunoglobulin mu chain.
    Cell. 1980 Jun;20(2):303-12 PMID: 6771019
  40. RNA synthesis and coding capacity of polyadenylated and nonpolyadenylated mRNA from cultures of differentiating Drosophila melanogaster myoblasts.
    Dev Biol. 1980 Oct;79(2):388-98 PMID: 6775993
  41. Identification of recombinant phages containing sequences from different rat myosin heavy chain genes.
    Nucleic Acids Res. 1980 May 24;8(10):2133-46 PMID: 7433088
  42. A cloning vehicle suitable for strand separation.
    Gene. 1980 Oct;11(1-2):109-15 PMID: 6254848
  43. A single mouse alpha-amylase gene specifies two different tissue-specific mRNAs.
    Cell. 1981 Feb;23(2):451-8 PMID: 6162570
  44. Regulation of myosin self-assembly: phosphorylation of Dictyostelium heavy chain inhibits formation of thick filaments.
    Proc Natl Acad Sci U S A. 1980 Dec;77(12):7292-6 PMID: 6452632
  45. Dictyostelium myosin: characterization of chymotryptic fragments and localization of the heavy-chain phosphorylation site.
    J Cell Biol. 1981 Apr;89(1):104-8 PMID: 7228895
  46. The actin genes of Drosophila: protein coding regions are highly conserved but intron positions are not.
    Cell. 1981 Apr;24(1):107-16 PMID: 6263481
  47. The sequence 5'-AAUAAA-3'forms parts of the recognition site for polyadenylation of late SV40 mRNAs.
    Cell. 1981 Apr;24(1):251-60 PMID: 6113054
  48. Characterization of the branch site in lariat RNAs produced by splicing of mRNA precursors.
    Nature. 1985 Feb 14-20;313(6003):552-7 PMID: 2578627
  49. Developmental variations in the splicing pattern of transcripts from the Drosophila gene encoding myosin alkali light chain result in different carboxyl-terminal amino acid sequences.
    Proc Natl Acad Sci U S A. 1985 Jan;82(2):449-53 PMID: 2982157
  50. Intricate combinatorial patterns of exon splicing generate multiple regulated troponin T isoforms from a single gene.
    Cell. 1985 May;41(1):67-82 PMID: 2986851
  51. Controlling roles for snurps.
    Nature. 1985 Jul 11-17;316(6024):105-6 PMID: 3159968
  52. Sequence analysis of mutations that affect the synthesis, assembly and enzymatic activity of the unc-54 myosin heavy chain of Caenorhabditis elegans.
    J Mol Biol. 1985 Jun 25;183(4):543-51 PMID: 4020869
  53. Intron splicing: a conserved internal signal in introns of Drosophila pre-mRNAs.
    Nucleic Acids Res. 1985 Jul 11;13(13):4971-81 PMID: 2410858
  54. A single cardiac troponin T gene generates embryonic and adult isoforms via developmentally regulated alternate splicing.
    J Biol Chem. 1985 Sep 15;260(20):11140-8 PMID: 2993302
  55. Novel deletion mutants that enhance a distant upstream 5' splice in the E3 transcription unit of adenovirus 2.
    Nucleic Acids Res. 1985 Aug 26;13(16):5771-88 PMID: 2412208
  56. Role of the 3' splice site consensus sequence in mammalian pre-mRNA splicing.
    Nature. 1985 Oct 24-30;317(6039):732-4 PMID: 4058579
  57. Alternative splicing caused by RNA secondary structure.
    Cell. 1985 Dec;43(3 Pt 2):667-76 PMID: 4075405
  58. Mutations of the Drosophila myosin heavy-chain gene: effects on transcription, myosin accumulation, and muscle function.
    Proc Natl Acad Sci U S A. 1986 Mar;83(5):1393-7 PMID: 3006049
  59. Alcohol dehydrogenase gene of Drosophila melanogaster: relationship of intervening sequences to functional domains in the protein.
    Proc Natl Acad Sci U S A. 1981 May;78(5):2717-21 PMID: 6789320
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.6.7.2511-2519.1986
Published
1986-07-00
Pages
2511-9
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC367805
Subset
IM
Grants
NIGMS NIH HHS · GM26706 · United States
NIGMS NIH HHS · GM32443 · United States
Databases
GENBANK
M13360
External Links
PubMed source DOI: 10.1128/mcb.6.7.2511-2519.1986 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2