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PMID: 2432013 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A murine monoclonal antibody specific for the outer core oligosaccharide of Salmonella lipopolysaccharide.

Infection and immunity ·Vol. 55 ·No. 1 ·1987-01-00 ·Pages 211-6

Tsang RS, Chan KH, Chau PY, Wan KC, Ng MH, Schlecht S

Abstract

Immunoglobulin G3 murine monoclonal antibody T6 specific for the lipopolysaccharide of Salmonella O serogroups A to E was established. By using R mutants of Salmonella spp., Escherichia coli, and Shigella spp., the major reactive epitope with T6 was tentatively identified as the terminal disaccharide, N-acetylglucosamine 1.2----alpha glucose, of the core oligosaccharide. T6 was reactive with 10 clinical isolates of each of the Salmonella O serogroups A to E but not with 58 isolates of other gram-negative bacteria. Its selective reactivity against Salmonella spp. renders T6 a potentially more useful reagent than the conventional polyvalent serum for the identification of Salmonella spp. It may also serve as a useful molecular tool for the study of the outer core structure of all Salmonella and related species.

MeSH Terms
Antibodies, Bacterial/immunology Antibodies, Monoclonal/immunology Antibody Specificity Antigens, Bacterial/immunology Carbohydrate Sequence Epitopes Hot Temperature Lipopolysaccharides/immunology Polysaccharides, Bacterial/immunology Radioimmunoassay Salmonella/immunology Salmonella typhimurium/immunology
Chemicals
Antibodies, Bacterial Antibodies, Monoclonal Antigens, Bacterial Epitopes Lipopolysaccharides Polysaccharides, Bacterial
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Tsang R S
Chan K H
Chau P Y
Wan K C
Ng M H
Schlecht S
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23 references, click to expand
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.55.1.211-216.1987
Published
1987-01-00
Pages
211-6
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC260304
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.55.1.211-216.1987 PMC Full Text View journal details
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