Comparison of fluorescent-antibody methods and enrichment serology for the detection of Salmonella.

Applied microbiology ·Vol. 27 ·No. 2 ·1974-02-00 ·Pages 324-8

Mohr HK, Trenk HL, Yeterian M

Abstract

Four rapid methods for detection of Salmonella, (i) the conventional fluorescent-antibody (FA) technique, (ii) a rapid direct FA technique, (iii) microcolony FA, and (iv) enrichment serology (ES), were compared with conventional cultural procedures. A total of 347 subsamples representing 16 different food prototypes, alleged to be naturally contaminated with Salmonella, were analyzed. From these samples, 52 were found to contain Salmonella by cultural methods. Conventional FA identified all 52 culturally positive samples, ES identified 51, microcolony FA identified 48, and the rapid FA method identified 34. The number of false-positive samples for each procedure was: ES-selenite, 7; tetrathionate, 8; rapid FA, 26; microcolony FA, 33; conventional FA-selenite, 27; tetrathionate, 26. Tetrathionate enrichment was found to be superior to selenite for Salmonella recovery from most foods, but the concurrent use of both media allowed maximum recovery.

MeSH Terms

Bacteriological Techniques Culture Media Evaluation Studies as Topic False Negative Reactions False Positive Reactions Fluorescent Antibody Technique Food Contamination Food Microbiology Methods Salmonella/growth & development,isolation & purification Selenium Thiosulfates

Chemicals

Culture Media Thiosulfates Selenium

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Mohr H K

Trenk H L

Yeterian M

References (10)

10 references, click to expand

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Article Info

Journal

Applied microbiology

Abbr.

Appl Microbiol

ISSN

0003-6919

DOI

10.1128/am.27.2.324-328.1974

Published

1974-02-00

Pages

324-8

Language

English

Region

United States

NLM ID

7605802

PMCID

PMC380028

Subset

External Links

PubMed source DOI: 10.1128/am.27.2.324-328.1974 PMC Full Text

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Comparison of fluorescent-antibody methods and enrichment serology for the detection of Salmonella.

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