Rapid, direct fluorescent-antibody method for the detection of salmonellae in food and feeds.

Applied microbiology ·Vol. 24 ·No. 4 ·1972-10-00 ·Pages 645-9

Insalata NF, Mahnke CW, Dunlap WG

Abstract

An improved immunofluorescent-antibody (FA) method for the detection of salmonellae in foods and feeds was developed. This FA method combines a rapid cultural phase and a serological phase that allow for propagation of salmonellae in a minimum time, employing the industrial 8-hr work day as a guide. Two hundred fifty naturally contaminated human food and animal feed samples, representing 647 trials, were tested by the FA method. A total of 18 different food and feed samples was used. The method used by the Association of Official Analytical Chemists (AOAC) for the detection of salmonellae was the control method. The percent agreement when comparing the FA slide method to the AOAC method ranged from 87.1 to 95.3%, depending upon the conjugated antisera used in comparative studies.

MeSH Terms

Animal Feed Antigens, Bacterial/analysis Culture Media Evaluation Studies as Topic Fluorescent Antibody Technique Food Contamination Food Microbiology Immune Sera Methods Microscopy, Fluorescence Salmonella/immunology,isolation & purification Salmonella typhimurium/immunology,isolation & purification Time Factors

Chemicals

Antigens, Bacterial Culture Media Immune Sera

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Insalata N F

Mahnke C W

Dunlap W G

References (10)

10 references, click to expand

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A direct fluorescent antibody test for Salmonella.
Arch Environ Health. 1969 Dec;19(6):876-81 PMID: 4900689
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Appl Microbiol. 1969 Nov;18(5):708-13 PMID: 4905601
An evaluation of a fluorescent antibody technique for the detection of Salmonellae in animal by-products, feeds, and tissues.
J Am Vet Med Assoc. 1970 Dec 1;157(11):1898-900 PMID: 4922195
One-day fluorescent-antibody procedure for detecting salmonellae in frozen and dried foods.
Appl Microbiol. 1970 Dec;20(6):977-83 PMID: 4923810
Preparation and testing of polyvalent conjugates for fluorescent-antibody detection of salmonellae.
Appl Microbiol. 1971 Nov;22(5):876-84 PMID: 4943590
Immunofluorescence technique for detection of Salmonellae in tissues of swine.
Am J Vet Res. 1972 Feb;33(2):445-7 PMID: 4551086
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Article Info

Journal

Applied microbiology

Abbr.

Appl Microbiol

ISSN

0003-6919

DOI

10.1128/am.24.4.645-649.1972

Published

1972-10-00

Pages

645-9

Language

English

Region

United States

NLM ID

7605802

PMCID

PMC380629

Subset

External Links

PubMed source DOI: 10.1128/am.24.4.645-649.1972 PMC Full Text

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