Immunofluorescent staining of Salmonella species with flagellar sera.

Applied microbiology ·Vol. 18 ·No. 4 ·1969-10-00 ·Pages 612-7

Goepfert JM, Hicks R

Abstract

Salmonellae stained with flagellar sera by either the direct or indirect fluorescent-antibody technique fluoresced at the cell surface. Investigations showed that fimbriae, flagellar material, and capsule antigens were not the participating antigens in this reaction. Cell surface staining was inhibited by mannose but was unaffected by glucose, galactose, lactose, maltose, and sucrose. Specific absorption of the flagellar sera by intact cells and purified somatic antigen showed that O antigens were the site of the surface staining. The advantages of using flagellar sera in fluorescent-antibody screening of materials for the presence of salmonellae are discussed.

MeSH Terms

Animal Feed Antigens Carbohydrate Metabolism Culture Media Flagella/immunology Fluorescent Antibody Technique Food Microbiology Salmonella/immunology,isolation & purification Serotyping Staining and Labeling

Chemicals

Antigens Culture Media

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Goepfert J M

Hicks R

References (10)

10 references, click to expand

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Immunofluorescence technique for the detection of salmonellae in various foods.
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Immunochemistry of O and R antigens of Salmonella and related Enterobacteriaceae.
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Accelerated procedure for Salmonella detection in dried foods and feeds involving only borth cultures and serological reactions.
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DETECTION OF SALMONELLA IN EGGS AND EGG PRODUCTS WITH FLUORESCENT ANTIBODY.
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Article Info

Journal

Applied microbiology

Abbr.

Appl Microbiol

ISSN

0003-6919

DOI

10.1128/am.18.4.612-617.1969

Published

1969-10-00

Pages

612-7

Language

English

Region

United States

NLM ID

7605802

PMCID

PMC378046

Subset

External Links

PubMed source DOI: 10.1128/am.18.4.612-617.1969 PMC Full Text

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