Expression of a murine polyclonal T cell receptor marker correlates with the use of specific members of the V beta 8 gene segment subfamily.
A series of murine T lymphocyte clones were examined for reactivity with the KJ16-133 and F23.1 mAbs. Clones that were KJ16-133+,F23.1+ and KJ16-133-,F23.1+ were identified, but no KJ16-133+,F23.1- clones were observed. Within our panel of clones, therefore, the KJ16-133 antibody identifies a subset of F23.1+ cells. All F23.1+ clones examined express members of the V beta 8 subfamily of beta chain variable region genes; clones expressing V beta 8.1 or V beta 8.2 reacted with both KJ16-133 and F23.1, while clones expressing V beta 8.3 reacted only with F23.1. Thus, the differential reactivity of the KJ16-133 and F23.1 antibodies with cloned T cells correlates with the V beta gene expression of each clone. Reactivity with these antibodies should therefore be of utility for predicting the V beta gene expression in some T cell clones.
13 references, click to expand
-
Characterization of a murine monoclonal antibody specific for an allotypic determinant on T cell antigen receptor.J Immunol. 1985 Jun;134(6):3994-4000 PMID: 2580901
-
Detection of specific sequences among DNA fragments separated by gel electrophoresis.J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
-
The major histocompatibility complex-restricted antigen receptor on T cells: the genetics of expression of an allotype.J Immunol. 1985 Sep;135(3):2176-82 PMID: 2410514
-
T-cell receptor beta-chain expression: dependence on relatively few variable region genes.Science. 1985 Aug 9;229(4713):566-70 PMID: 3875151
-
The murine T-cell receptor uses a limited repertoire of expressed V beta gene segments.Nature. 1985 Aug 8-14;316(6028):517-23 PMID: 2412120
-
Murine T-cell receptor mutants with deletions of beta-chain variable region genes.Proc Natl Acad Sci U S A. 1986 Feb;83(3):767-71 PMID: 3456168
-
The antigen-specific, major histocompatibility complex-restricted receptor on T cells. VI. An antibody to a receptor allotype.J Exp Med. 1984 Aug 1;160(2):452-71 PMID: 6206178
-
Fine specificity and antigen receptor expression among influenza virus-specific cytolytic T lymphocyte clones.J Immunol. 1986 Aug 1;137(3):995-1002 PMID: 2424996
-
V beta gene polymorphism and a major polyclonal T cell receptor idiotype.Cell. 1985 Aug;42(1):89-92 PMID: 2410144
-
Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.Biochemistry. 1979 Nov 27;18(24):5294-9 PMID: 518835
-
Identification of a third type of lambda light chain in mouse immunoglobulins.Proc Natl Acad Sci U S A. 1981 Jan;78(1):569-73 PMID: 6165998
-
Heterogeneity and specificity of cloned lines of influenza-virus specific cytotoxic T lymphocytes.J Exp Med. 1981 Apr 1;153(4):910-23 PMID: 6166708
-
T-cell receptor variable region gene usage in T-cell populations.Proc Natl Acad Sci U S A. 1986 Jun;83(11):3987-91 PMID: 3487085
Contact
No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong
Qilu Normal University · Genelibs Bioinformatics Lab
750 Shunhua Rd, Jinan
2F, Bldg F, University Science Park
Tel: 0531-88819269
WeChat Official Account
Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.
Business Email
E-mail: [email protected]
Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2
