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PMID: 2432399 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Kinetoplast DNA of Bodo caudatus: a noncatenated structure.

Molecular and cellular biology ·Vol. 6 ·No. 12 ·1986-12-00 ·Pages 4372-8

Hajduk SL, Siqueira AM, Vickerman K

Abstract

The kinetoplast DNA (kDNA) of trypanosomes and other parasitic members of the order Kinetoplastida is organized as a complex network containing thousands of catenated circular DNA molecules. We found that the kDNA of a free-living kinetoplastida, Bodo caudatus, exists as a noncatenated structure. The kDNA of B. caudatus represents about 40% of the total cellular DNA, and the major components of this DNA are large circles of 10 and 12 kilobases (kb). Our results indicate that these circles are analogous to trypanosome kDNA minicircles despite their large size and noncatenated form. The kDNA of B. caudatus also contains a minor component of 19 kb which is transcribed. The 19-kb molecules are probably analogous to the maxicircles of trypanosomes. The properties of the B. caudatus kDNA suggest that the catenated network structure of trypanosome kDNA is not required for maxicircle segregation during kinetoplast division or for the expression of the maxicircle genome.

MeSH Terms
Animals DNA Restriction Enzymes DNA, Circular/genetics,isolation & purification,ultrastructure DNA, Kinetoplast Microscopy, Electron Molecular Weight Nucleic Acid Hybridization RNA/genetics,isolation & purification Trypanosoma/genetics
Chemicals
DNA, Circular DNA, Kinetoplast RNA DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Hajduk S L
Siqueira A M
Vickerman K
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26 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.6.12.4372-4378.1986
Published
1986-12-00
Pages
4372-8
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC367219
Subset
IM
Grants
NIAID NIH HHS · AI 21401 · United States
NIGMS NIH HHS · GM 27608 · United States
External Links
PubMed source DOI: 10.1128/mcb.6.12.4372-4378.1986 PMC Full Text View journal details
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