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PMID: 6306559 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

The major transcripts of the kinetoplast DNA of Trypanosoma brucei are very small ribosomal RNAs.

Nucleic acids research ·Vol. 11 ·No. 1 ·1983-01-11 ·Pages 105-25

Eperon IC, Janssen JW, Hoeijmakers JH, Borst P

Abstract

The nucleotide sequence has been determined of a 2.2 kb segment of kinetoplast DNA, which encodes the major mitochondrial transcripts (12S and 9S) of Trypanosoma brucei. The sequence shows that the 12S RNA is a large subunit rRNA, although sufficiently unusual for resistance to chloramphenicol to be predicted. The 9S RNA has little homology with other rRNAs, but a possible secondary structure is not unlike that of the 2.5-fold larger E. coli 16S rRNA. We conclude that the 12S RNA (about 1230 nucleotides) and the 9S RNA (about 640 nucleotides) are the smallest homologues of the E. coli 23S and 16S rRNAs yet observed.

MeSH Terms
Animals Base Composition Base Sequence Chloramphenicol/pharmacology DNA Restriction Enzymes Drug Resistance Molecular Weight Nucleic Acid Conformation RNA, Ribosomal/genetics Species Specificity Transcription, Genetic Trypanosoma brucei brucei/drug effects,genetics
Chemicals
RNA, Ribosomal Chloramphenicol DNA Restriction Enzymes
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Eperon I C
Janssen J W
Hoeijmakers J H
Borst P
References (75)
75 references, click to expand
  1. Complete nucleotide sequence of a 23S ribosomal RNA gene from Escherichia coli.
    Proc Natl Acad Sci U S A. 1980 Jan;77(1):201-4 PMID: 6153795
  2. Sequence of the intron and flanking exons of the mitochondrial 21S rRNA gene of yeast strains having different alleles at the omega and rib-1 loci.
    Cell. 1980 May;20(1):185-97 PMID: 6156002
  3. Distinctive sequence of human mitochondrial ribosomal RNA genes.
    Nature. 1980 Jul 31;286(5772):460-7 PMID: 6157106
  4. Precise localization and nucleotide sequence of the two mouse mitochondrial rRNA genes and three immediately adjacent novel tRNA genes.
    Cell. 1980 Nov;22(1 Pt 1):157-70 PMID: 7428037
  5. Secondary structure model for bacterial 16S ribosomal RNA: phylogenetic, enzymatic and chemical evidence.
    Nucleic Acids Res. 1980 May 24;8(10):2275-93 PMID: 6159576
  6. A computer program to search for tRNA genes.
    Nucleic Acids Res. 1980 Feb 25;8(4):817-25 PMID: 7433117
  7. A short primer for sequencing DNA cloned in the single-stranded phage vector M13mp2.
    Nucleic Acids Res. 1980 Apr 25;8(8):1731-43 PMID: 6253946
  8. 6.5 S RNA; preliminary characterisation of unusual small RNAs in Trypanosoma brucei.
    Mol Biochem Parasitol. 1980 Apr;1(2):91-6 PMID: 7442711
  9. An experimentally-derived model for the secondary structure of the 16S ribosomal RNA from Escherichia coli.
    Nucleic Acids Res. 1980 Jun 11;8(11):2377-95 PMID: 6160459
  10. The structure of the yeast ribosomal RNA genes. I. The complete nucleotide sequence of the 18S ribosomal RNA gene from Saccharomyces cerevisiae.
    Nucleic Acids Res. 1980 Dec 11;8(23):5779-94 PMID: 7008030
  11. Cultivation of infective forms of Trypanosoma congolense from trypanosomes in the proboscis of Glossina morsitans.
    Parasitology. 1981 Feb;82(1):81-95 PMID: 7208105
  12. The secondary structure of the protein L1 binding region of ribosomal 23S RNA. Homologies with putative secondary structures of the L11 mRNA and of a region of mitochondrial 16S rRNA.
    Nucleic Acids Res. 1981 Jan 24;9(2):293-307 PMID: 7010313
  13. A system for shotgun DNA sequencing.
    Nucleic Acids Res. 1981 Jan 24;9(2):309-21 PMID: 6259625
  14. Methylated regions of hamster mitochondrial ribosomal RNA: structural and functional correlates.
    Nucleic Acids Res. 1981 Jan 24;9(2):323-37 PMID: 6782552
  15. Secondary structure of 16S ribosomal RNA.
    Science. 1981 Apr 24;212(4493):403-11 PMID: 6163215
  16. Variations in maxi-circle and mini-circle sequences in kinetoplast DNAs from different Trypanosoma brucei strains.
    Biochim Biophys Acta. 1980 Dec 11;610(2):197-210 PMID: 6260147
  17. The 3' terminus of the large ribosomal subunit ("17S") RNA from hamster mitochondria is ragged and oligoadenylated.
    Cell. 1981 Jan;23(1):271-8 PMID: 7214525
  18. Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing.
    J Mol Biol. 1980 Oct 25;143(2):161-78 PMID: 6260957
  19. Inhibitory effects of chloramphenicol isomers and other antibiotics on protein synthesis and respiration in procyclic Trypanosoma brucei brucei.
    Mol Biochem Parasitol. 1981 Feb;2(3-4):235-55 PMID: 7219444
  20. Altered ribosomal RNA genes in mitochondria from mammalian cells with chloramphenicol resistance.
    Nature. 1981 Apr 16;290(5807):607-8 PMID: 7219548
  21. Feedback regulation of ribosomal protein gene expression in Escherichia coli: structural homology of ribosomal RNA and ribosomal protein MRNA.
    Proc Natl Acad Sci U S A. 1980 Dec;77(12):7084-8 PMID: 7012833
  22. [Nucleotide sequence of the gene for the mitochondrial 15S ribosomal RNA of yeast].
    C R Seances Acad Sci D. 1980 Dec 8;291(12):933-6 PMID: 6261980
  23. Nucleotide sequence of Xenopus laevis 18S ribosomal RNA inferred from gene sequence.
    Nature. 1981 May 21;291(5812):205-8 PMID: 7015146
  24. A putative precursor for the small ribosomal RNA from mitochondria of Saccharomyces cerevisiae.
    Nucleic Acids Res. 1981 Mar 25;9(6):1351-64 PMID: 6262728
  25. Rapid synthesis of oligodeoxyribonucleotides VI. Efficient, mechanised synthesis of heptadecadeoxyribonucleotides by an improved solid phase phosphotriester route.
    Nucleic Acids Res. 1981 Apr 10;9(7):1691-706 PMID: 7232224
  26. Nucleotide sequence of a ribosomal RNA gene intron from slime mold Physarum polycephalum.
    Proc Natl Acad Sci U S A. 1981 Mar;78(3):1376-80 PMID: 6262791
  27. Cloning and expression of Trypanosoma brucei kinetoplast DNA in Escherichia coli.
    Gene. 1980 Dec;12(3-4):223-34 PMID: 7019010
  28. The sequence of the ribosomal 16S RNA from Proteus vulgaris. Sequence comparison with E. coli 16S RNA and its use in secondary model building.
    Nucleic Acids Res. 1981 May 25;9(10):2325-33 PMID: 7019853
  29. Specific binding of a prokaryotic ribosomal protein to a eukaryotic ribosomal RNA: implications for evolution and autoregulation.
    Proc Natl Acad Sci U S A. 1981 May;78(5):2722-6 PMID: 6265904
  30. Mitochondrial DNA of chloramphenicol-resistant mouse cells contains a single nucleotide change in the region encoding the 3' end of the large ribosomal RNA.
    Proc Natl Acad Sci U S A. 1981 Jun;78(6):3789-93 PMID: 6943583
  31. Transcription of kinetoplast DNA in Trypanosoma brucei bloodstream and culture forms.
    Plasmid. 1981 May;5(3):329-50 PMID: 6267635
  32. The rRNA operon from Zea mays chloroplasts: nucleotide sequence of 23S rDNA and its homology with E.coli 23S rDNA.
    Nucleic Acids Res. 1981 Jun 25;9(12):2853-69 PMID: 7024906
  33. Shotgun DNA sequencing using cloned DNase I-generated fragments.
    Nucleic Acids Res. 1981 Jul 10;9(13):3015-27 PMID: 6269069
  34. Secondary structure of the large subunit ribosomal RNA from Escherichia coli, Zea mays chloroplast, and human and mouse mitochondrial ribosomes.
    Nucleic Acids Res. 1981 Jul 24;9(14):3287-306 PMID: 7024913
  35. Secondary structure comparisons between small subunit ribosomal RNA molecules from six different species.
    Nucleic Acids Res. 1981 Aug 11;9(15):3621-40 PMID: 7024918
  36. Primary and secondary structures of Escherichia coli MRE 600 23S ribosomal RNA. Comparison with models of secondary structure for maize chloroplast 23S rRNA and for large portions of mouse and human 16S mitochondrial rRNAs.
    Nucleic Acids Res. 1981 Sep 11;9(17):4303-24 PMID: 6170936
  37. Structural organization of the 16S ribosomal RNA from E. coli. Topography and secondary structure.
    Nucleic Acids Res. 1981 May 11;9(9):2153-72 PMID: 6170942
  38. Different nucleotide changes in the large rRNA gene of the mitochondrial DNA confer chloramphenicol resistance on two human cell lines.
    Nucleic Acids Res. 1981 Nov 11;9(21):5785-95 PMID: 6273808
  39. The primary and secondary structure of yeast 26S rRNA.
    Nucleic Acids Res. 1981 Dec 21;9(24):6935-52 PMID: 7335496
  40. Complete sequence of bovine mitochondrial DNA. Conserved features of the mammalian mitochondrial genome.
    J Mol Biol. 1982 Apr 25;156(4):683-717 PMID: 7120390
  41. Unusual pattern of ribonucleic acid components in the ribosome of Crithidia fasciculata, a trypanosomatid protozoan.
    Mol Cell Biol. 1981 Apr;1(4):347-57 PMID: 6965102
  42. Physical properties and protein constituents fo cytoplasmic and mitochondrial ribosomes of Xenopus laevis.
    J Biol Chem. 1974 Aug 25;249(16):5108-18 PMID: 4853633
  43. Eukaryotic ribosomal proteins: the number of proteins in the subunits and their isoelectric points.
    FEBS Lett. 1974 Sep 1;45(1):299-303 PMID: 4606528
  44. Affinity labelling of 23-S ribosomal RNA in the active centre of Escherichia coli peptidyl transferase.
    Eur J Biochem. 1974 Dec 2;49(3):539-44 PMID: 4613553
  45. Conservation of primary structure in 16S ribosomal RNA.
    Nature. 1975 Mar 6;254(5495):83-6 PMID: 1089909
  46. Are 50% of all cellular proteins synthesized on mitochondrial ribosomes in Crithidia luciliae?
    Biochim Biophys Acta. 1975 Apr 16;390(1):78-81 PMID: 1125314
  47. Mitochondrial and cytoplasmic ribosomes and their activity in blood and culture form Trypanosoma brucei.
    J Cell Biol. 1975 Apr;65(1):103-11 PMID: 1127006
  48. The importance of Escherichia coli ribosomal proteins L1, L11 and L16 for the association of ribosomal subunits and the formation of the 70-S initiation complex.
    Eur J Biochem. 1975 Oct 15;58(2):501-10 PMID: 1102306
  49. Specificity of substrate recognition by the EcoRI restriction endonuclease.
    Proc Natl Acad Sci U S A. 1975 Sep;72(9):3310-4 PMID: 242001
  50. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  51. Mammalian mitochondrial ribosomes:characterization of ribosomal proteins by two-dimensional gel electrophoresis.
    FEBS Lett. 1976 Mar 1;62(3):259-62 PMID: 1278367
  52. Binding of aminoacyl-tRNA to reconstituted subparticles of Escherichia coli large ribosomal subunits.
    Eur J Biochem. 1976 Aug 16;67(2):373-8 PMID: 786630
  53. RNA sequences associated with proteins L1, L9, and L5, L18, L25, in ribonucleoprotein fragments isolated from the 50-S subunit of Escherichia coli ribosomes.
    Eur J Biochem. 1976 Nov 15;70(2):483-92 PMID: 827440
  54. Characterization of the regions from E. coli 16 S RNA covalently linked to ribosomal proteins S4 and S20 after ultraviolet irradiation.
    FEBS Lett. 1977 Jun 15;78(2):261-6 PMID: 885249
  55. Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids.
    Cell. 1977 Nov;12(3):721-32 PMID: 922889
  56. Analysis of mitochondrial ribosomal proteins of Saccharomyces cerevisiae by two dimensional polyacrylamide gel electrophoresis.
    Mol Gen Genet. 1977 Sep 21;155(1):27-34 PMID: 337115
  57. Sequence data handling by computer.
    Nucleic Acids Res. 1977 Nov;4(11):4037-51 PMID: 593900
  58. Sequence at the site of attachment of an affinity-label derivative of puromycin on 23-S ribosomal RNA of Escherichia coli ribosomes.
    Eur J Biochem. 1978 Jan 2;82(1):225-34 PMID: 413716
  59. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  60. Methylation of single-stranded DNA in vitro introduces new restriction endonuclease cleavage sites.
    Nature. 1978 Mar 23;272(5651):375-7 PMID: 416358
  61. Kinetoplast RNA of Leishmania tarentolae.
    Cell. 1978 May;14(1):169-78 PMID: 667932
  62. Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli.
    Proc Natl Acad Sci U S A. 1978 Oct;75(10):4801-5 PMID: 368799
  63. Isolation and characterization of kinetoplast DNA and RNA of Phytomonas davidi.
    Plasmid. 1978 Jun;1(3):297-315 PMID: 748951
  64. Photochemical cross-linking of tRNALys and tRNA2Glu to 16 S RNA at the P site of Escherichia coli ribosomes.
    J Biol Chem. 1979 Jun 10;254(11):4745-9 PMID: 108282
  65. Fine structure of the 21S ribosomal RNA region on yeast mitochondrial DNA. II. The organization of sequences in petite mitochondrial DNAs carrying genetic markers from the 21S region.
    Mol Gen Genet. 1979 Jan 11;168(3):251-77 PMID: 374988
  66. Laboratory of Physiological Chemistry, State University Groningen, Netherlands.
    Nucleic Acids Res. 1979;6(5):1791-803 PMID: 156351
  67. Rapid evolution of animal mitochondrial DNA.
    Proc Natl Acad Sci U S A. 1979 Apr;76(4):1967-71 PMID: 109836
  68. Split gene for mitochondrial 24S ribosomal RNA of Neurospora crassa.
    Cell. 1979 May;17(1):191-200 PMID: 156584
  69. Mitochondrial ribosomes of Neurospora crassa: isolation, analysis, and use.
    Methods Enzymol. 1979;56:79-92 PMID: 156869
  70. Kinetoplast DNA.
    Plasmid. 1979 Jan;2(1):20-40 PMID: 382201
  71. Organization of tRNA and rRNA genes in N. crassa mitochondria: intervening sequence in the large rRNA gene and strand distribution of the RNA genes.
    Cell. 1979 Jul;17(3):583-95 PMID: 157815
  72. Protection of specific sites in 23 S and 5 S RNA from chemical modification by association of 30 S and 50 S ribosomes.
    J Mol Biol. 1979 Jun 5;130(4):421-32 PMID: 384002
  73. Mechanism of ribosomal subunit association: discrimination of specific sites in 16 S RNA essential for association activity.
    J Mol Biol. 1979 Jun 5;130(4):433-49 PMID: 384003
  74. Structure analysis at the ends of the intervening DNA sequences in the chloroplast 23S ribosomal genes of C. reinhardii.
    Cell. 1979 Sep;18(1):55-60 PMID: 509525
  75. Trans-complementable copy-number mutants of plasmid ColE1.
    Nature. 1980 Jan 10;283(5743):216-8 PMID: 7350544
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/11.1.105
Published
1983-01-11
Pages
105-25
Language
English
Region
England
NLM ID
0411011
PMCID
PMC325693
Subset
IM
Databases
GENBANK
J01457
External Links
PubMed source DOI: 10.1093/nar/11.1.105 PMC Full Text View journal details
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