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PMID: 6262791 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Nucleotide sequence of a ribosomal RNA gene intron from slime mold Physarum polycephalum.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 78 ·No. 3 ·1981-03-00 ·Pages 1376-80

Nomiyama H, Sakaki Y, Takagi Y

Abstract

The Physarum polycephalum 26S ribosomal RNA gene contains two intervening sequences (introns). The DNA sequence of one of these introns was analyzed together with that of its flanking regions (exons). In addition, the nucleotide sequence of the corresponding region of the reverse transcript of 26S rRNA was determined, and from comparisons of both sequences the precise location and size of the intron were determined. Our findings, when compared with those for Tetrahymena and Chlamydomonas rRNA gene introns, led to the conclusion that certain characteristics exist near the ends of these introns. (i) An exon ends in T at the exon/intron junction and an intron ends in G at the intron/exon junction in all cases. (ii) For each intron, direct repeats several nucleotides long are present 5 to approximately 30 nucleotides upstream from both the exon/intron and intron/exon junctions.

MeSH Terms
Base Sequence DNA Restriction Enzymes DNA, Fungal/genetics Genes Nucleic Acid Hybridization Oligodeoxyribonucleotides/analysis Physarum/genetics RNA, Ribosomal/genetics
Chemicals
DNA, Fungal Oligodeoxyribonucleotides RNA, Ribosomal DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Nomiyama H
Sakaki Y
Takagi Y
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36 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.78.3.1376
Published
1981-03-00
Pages
1376-80
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC319133
Subset
IM
Databases
GENBANK
J01287, V01160
External Links
PubMed source DOI: 10.1073/pnas.78.3.1376 PMC Full Text View journal details
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