The intervening sequence of a mouse beta-globin gene is transcribed within the 15S beta-globin mRNA precursor.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 75 ·No. 3 ·1978-03-00 ·Pages 1309-13

Tilghman SM, Curtis PJ, Tiemeier DC, Leder P, Weissmann C

Abstract

Mouse beta-globin in encoded in a discontinuous structural gene interrupted by a 550-base pair intervening sequence of DNA. Correspondingly, the mature beta-globin mRNA appears to be synthesized via a 15S precursor, the length of which roughly equals the total length of the coding and intervening sequences of the beta-globin gene. Using the electron microscope to visualize hybrid structures formed between this gene and the purified 15S beta-globin mRNA precursor, we show that the intervening sequence is present within the larger precursor molecule. This finding suggests that the precursor mRNA is processed through the removal and rejoining of internal RNA sequences.

MeSH Terms

Cell Line Chromosome Mapping DNA/genetics DNA, Recombinant Genes Globins/genetics Microscopy, Electron Molecular Weight Nucleic Acid Hybridization Nucleic Acid Precursors/genetics,isolation & purification RNA, Messenger/genetics

Chemicals

DNA, Recombinant Nucleic Acid Precursors RNA, Messenger Globins DNA

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Tilghman S M

Curtis P J

Tiemeier D C

Leder P

Weissmann C

References (27)

27 references, click to expand

Intervening sequence of DNA identified in the structural portion of a mouse beta-globin gene.
Proc Natl Acad Sci U S A. 1978 Feb;75(2):725-9 PMID: 273235
The rabbit beta-globin gene contains a large large insert in the coding sequence.
Cell. 1977 Dec;12(4):1097-108 PMID: 597859
Presence of a putative 15S precursor to beta-globin mRNA but not to alpha-globin mRNA in Friend cells.
Proc Natl Acad Sci U S A. 1977 Aug;74(8):3184-8 PMID: 410017
Cloning specific segments of the mammalian genome: bacteriophage lambda containing mouse globin and surrounding gene sequences.
Proc Natl Acad Sci U S A. 1977 Oct;74(10):4406-10 PMID: 270684
Purification of a putative precursor of globin messenger RNA from mouse nucleated erythroid cells.
Proc Natl Acad Sci U S A. 1977 Jan;74(1):178-82 PMID: 264673
Globin RNA precursor molecules: biosynthesis and process in erythroid cells.
Cell. 1977 Jul;11(3):641-50 PMID: 884738
Purification of globin messenger RNA from dimethylsulfoxide-induced Friend cells and detection of a putative globin messenger RNA precursor.
J Mol Biol. 1976 Oct 5;106(4):1067-75 PMID: 824454
A precursor of globin messenger RNA.
J Mol Biol. 1976 Sep 15;106(2):403-20 PMID: 978727
Organization and expression of early genes of simian virus 40.
Proc Natl Acad Sci U S A. 1978 Jan;75(1):117-21 PMID: 203926
Novel mechanism for RNA maturation: the leader sequences of simian virus 40 mRNA are not transcribed adjacent to the coding sequences.
Proc Natl Acad Sci U S A. 1977 Sep;74(9):3686-90 PMID: 198797
Comparison of the nucleotide sequence of the messenger RNA for the major structural protein of SV40 with the DNA sequence encoding the amino acids of the protein.
Nucleic Acids Res. 1977 Aug;4(8):2549-50 PMID: 198746
Variable and constant parts of the immunoglobulin light chain gene of a mouse myeloma cell are 1250 nontranslated bases apart.
Proc Natl Acad Sci U S A. 1977 Dec;74(12):5652-6 PMID: 414223
Sequence arrangement of the rDNA of Drosophila melanogaster.
Cell. 1977 Feb;10(2):213-4 PMID: 402223
The structural organization of ribosomal DNA in Drosophila melanogaster.
Cell. 1977 Feb;10(2):193-212 PMID: 402222
R loop mapping of the 18S and 28S sequences in the long and short repeating units of Drosophila melanogaster rDNA.
Cell. 1977 Feb;10(2):177-92 PMID: 402221
A novel arrangement of the 18S and 28S sequences in a repeating unit of Drosophila melanogaster rDNA.
Cell. 1977 Feb;10(2):167-76 PMID: 402220
Nucleotide sequence of a mutant eukaryotic gene: the yeast tyrosine-inserting ochre suppressor SUP4-o.
Proc Natl Acad Sci U S A. 1977 Dec;74(12):5453-7 PMID: 341157
Spliced segments at the 5' terminus of adenovirus 2 late mRNA.
Proc Natl Acad Sci U S A. 1977 Aug;74(8):3171-5 PMID: 269380
Intragenic DNA spacers interrupt the ovalbumin gene.
Proc Natl Acad Sci U S A. 1978 Mar;75(3):1299-303 PMID: 274719
Loop structures in hybrids of early RNA and the separated strands of adenovirus DNA.
Proc Natl Acad Sci U S A. 1977 Oct;74(10):4392-5 PMID: 270682
The presence of ovalbumin mRNA coding sequences in multiple restriction fragments of chicken DNA.
Nucleic Acids Res. 1977 Nov;4(11):3701-13 PMID: 563592
Purification and cloning of a mouse ribosomal gene fragment in coliphage lambda.
Gene. 1977;2(3-4):173-91 PMID: 608592
Ovalbumin gene is split in chicken DNA.
Nature. 1977 Nov 24;270(5635):314-9 PMID: 593351
Two adenovirus mRNAs have a common 5' terminal leader sequence encoded at least 10 kb upstream from their main coding regions.
Cell. 1977 Sep;12(1):9-21 PMID: 902321
An amazing sequence arrangement at the 5' ends of adenovirus 2 messenger RNA.
Cell. 1977 Sep;12(1):1-8 PMID: 902310
Isolation and characterization of simian virus 40 ribonucleic acid.
J Virol. 1972 Aug;10(2):193-201 PMID: 4342237
A quantitative assay for DNA-RNA hybrids with DNA immobilized on a membrane.
J Mol Biol. 1965 Jul;12(3):829-42 PMID: 4955314

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.75.3.1309

Published

1978-03-00

Pages

1309-13

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC411460

Subset

External Links

PubMed source DOI: 10.1073/pnas.75.3.1309 PMC Full Text View journal details

Analysis Services

The intervening sequence of a mouse beta-globin gene is transcribed within the 15S beta-globin mRNA precursor.

Contact

WeChat Official Account