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PMID: 2438367 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Use of synthetic peptides of influenza nucleoprotein to define epitopes recognized by class I-restricted cytotoxic T lymphocytes.

The Journal of experimental medicine ·Vol. 165 ·No. 6 ·1987-06-01 ·Pages 1508-23

Bastin J, Rothbard J, Davey J, Jones I, Townsend A

Abstract

The conserved epitopes of influenza nucleoprotein (NP) recognized by class I MHC-restricted CTL from CBA (H-2k) and C57BL/10 (H-2b) mice have been defined in vitro with synthetic peptides 50-63 and 365-379, respectively. Two Db-restricted clones were described that recognize different epitopes on peptide 365-379. Finally, the recognition of complete NP was shown to be approximately 200-fold less efficient than peptide in the cytotoxicity assay. These phenomena are closely related to results with class II-restricted T cells and they strengthen the hypothesis that influenza proteins are degraded in the infected cell before recognition by class I-restricted CTL.

MeSH Terms
Amino Acid Sequence Animals Cytotoxicity Tests, Immunologic Epitopes/analysis Female Histocompatibility Antigens/immunology Influenza A virus/immunology Mice Mice, Inbred C57BL Nucleoproteins/immunology T-Lymphocytes, Cytotoxic/immunology Viral Proteins/immunology
Chemicals
Epitopes Histocompatibility Antigens Nucleoproteins Viral Proteins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Bastin J
Rothbard J
Davey J
Jones I
Townsend A
References (42)
42 references, click to expand
  1. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
    Anal Biochem. 1976 May 7;72:248-54 PMID: 942051
  2. Cytotoxic T cells to type A influenza virus; viral hemagglutinin induces A-strain specificity while infected cells confer cross-reactive cytotoxicity.
    Eur J Immunol. 1977 Sep;7(9):630-5 PMID: 303568
  3. Nonrandom association of parental genes in influenza A virus recombinants.
    Virology. 1979 May;95(1):269-74 PMID: 442543
  4. Gene composition of high-yielding influenza vaccine strains obtained by recombination.
    J Infect Dis. 1980 Mar;141(3):362-5 PMID: 7365284
  5. Thymoma production of T cell growth factor (Interleukin 2).
    J Immunol. 1980 Dec;125(6):2555-8 PMID: 6968789
  6. Expression of influenza A virus internal antigens on the surface of infected P815 cells.
    J Immunol. 1981 May;126(5):1814-9 PMID: 7217668
  7. The structure of the gene encoding the nucleoprotein of human influenza virus A/PR/8/34.
    Virology. 1981 Oct 30;114(2):423-8 PMID: 7292985
  8. Rat brain Thy-1 glycoprotein. The amino acid sequence, disulphide bonds and an unusual hydrophobic region.
    Biochem J. 1981 Apr 1;195(1):15-30 PMID: 6118137
  9. The sequence of the nucleoprotein gene of human influenza A virus, strain A/NT/60/68.
    Nucleic Acids Res. 1982 Feb 11;10(3):1029-38 PMID: 6278431
  10. Recognition of Db and Kb gene products by influenza-specific cytotoxic T cells.
    Immunogenetics. 1983;17(3):283-94 PMID: 6601053
  11. The T lymphocyte response to cytochrome c. IV. Distinguishable sites on a peptide antigen which affect antigenic strength and memory.
    J Immunol. 1983 Jul;131(1):319-24 PMID: 6190913
  12. Antigen presentation: comments on its regulation and mechanism.
    J Immunol. 1984 Jan;132(1):1-5 PMID: 6228574
  13. Gene transfer of H-2 class II genes: antigen presentation by mouse fibroblast and hamster B-cell lines.
    Cell. 1984 Feb;36(2):319-27 PMID: 6607120
  14. Differential requirements for antigen processing by macrophages for lysozyme-specific T cell hybridomas.
    J Immunol. 1984 Mar;132(3):1077-9 PMID: 6607276
  15. A limited region within hen egg-white lysozyme serves as the focus for a diversity of T cell clones.
    J Immunol. 1984 Oct;133(4):2075-8 PMID: 6206149
  16. The influenza A virus nucleoprotein gene controls the induction of both subtype specific and cross-reactive cytotoxic T cells.
    J Exp Med. 1984 Aug 1;160(2):552-63 PMID: 6206181
  17. Cytotoxic T cell recognition of the influenza nucleoprotein and hemagglutinin expressed in transfected mouse L cells.
    Cell. 1984 Nov;39(1):13-25 PMID: 6091906
  18. Antigen conformation determines processing requirements for T-cell activation.
    Proc Natl Acad Sci U S A. 1984 Nov;81(21):6831-5 PMID: 6333686
  19. Specificity of cytotoxic T lymphocytes stimulated with influenza virus. Studies in mice and humans.
    Prog Allergy. 1985;36:10-43 PMID: 3881777
  20. Identification of the sequence responsible for the nuclear accumulation of the influenza virus nucleoprotein in Xenopus oocytes.
    Cell. 1985 Mar;40(3):667-75 PMID: 3838265
  21. Influenza A virus nucleoprotein is a major target antigen for cross-reactive anti-influenza A virus cytotoxic T lymphocytes.
    Proc Natl Acad Sci U S A. 1985 Mar;82(6):1785-9 PMID: 3872457
  22. Specificity of the T cell receptor: two different determinants are generated by the same peptide and the I-Ak molecule.
    J Immunol. 1985 Jul;135(1):368-73 PMID: 2582039
  23. Induction of regulatory T-lymphocyte responses by liposomes carrying major histocompatibility complex molecules and foreign antigen.
    Nature. 1985 May 23-29;315(6017):327-9 PMID: 3873617
  24. Cytotoxic T cells recognize fragments of the influenza nucleoprotein.
    Cell. 1985 Sep;42(2):457-67 PMID: 2411422
  25. Differential expression of influenza N protein and neuraminidase antigenic determinants in Escherichia coli.
    Gene. 1985;35(3):333-42 PMID: 2931323
  26. T-cell antigenic sites tend to be amphipathic structures.
    Proc Natl Acad Sci U S A. 1985 Oct;82(20):7048-52 PMID: 2413457
  27. Antigen-presenting function of the macrophage.
    Annu Rev Immunol. 1984;2:395-428 PMID: 6242349
  28. Differences in antigen presentation to MHC class I-and class II-restricted influenza virus-specific cytolytic T lymphocyte clones.
    J Exp Med. 1986 Apr 1;163(4):903-21 PMID: 3485173
  29. The epitopes of influenza nucleoprotein recognized by cytotoxic T lymphocytes can be defined with short synthetic peptides.
    Cell. 1986 Mar 28;44(6):959-68 PMID: 2420472
  30. Low responder MHC alleles for Tc recognition of influenza nucleoprotein.
    Immunogenetics. 1986;23(6):379-84 PMID: 3487504
  31. Fine specificity and antigen receptor expression among influenza virus-specific cytolytic T lymphocyte clones.
    J Immunol. 1986 Aug 1;137(3):995-1002 PMID: 2424996
  32. Influenza nucleoprotein-specific cytotoxic T-cell clones are protective in vivo.
    Immunology. 1986 Jul;58(3):417-20 PMID: 2426185
  33. Influenza virus-specific cytotoxic T-cell recognition: stimulation of nucleoprotein-specific clones with intact antigen.
    Immunology. 1986 Oct;59(2):173-80 PMID: 2429918
  34. HLA B37 determines an influenza A virus nucleoprotein epitope recognized by cytotoxic T lymphocytes.
    J Exp Med. 1986 Nov 1;164(5):1397-406 PMID: 2430041
  35. T-cell epitope of the autoantigen myelin basic protein that induces encephalomyelitis.
    Nature. 1986 Nov 20-26;324(6094):258-60 PMID: 2431317
  36. Interaction of peptide antigens and class II major histocompatibility complex antigens.
    Nature. 1986 Nov 20-26;324(6094):260-2 PMID: 2946957
  37. Cytotoxic T lymphocytes recognize influenza haemagglutinin that lacks a signal sequence.
    Nature. 1986 Dec 11-17;324(6097):575-7 PMID: 3491325
  38. H-2-restricted cytolytic T cells specific for HLA can recognize a synthetic HLA peptide.
    Nature. 1986 Dec 11-17;324(6097):578-9 PMID: 3491326
  39. Assembly of influenza ribonucleoprotein in vitro using recombinant nucleoprotein.
    Virology. 1987 Feb;156(2):396-403 PMID: 3811240
  40. Identification of viral molecules recognized by influenza-specific human cytotoxic T lymphocytes.
    J Exp Med. 1987 Feb 1;165(2):408-16 PMID: 3029268
  41. Anti-influenza virus cytotoxic T lymphocytes recognize the three viral polymerases and a nonstructural protein: responsiveness to individual viral antigens is major histocompatibility complex controlled.
    J Virol. 1987 Apr;61(4):1098-102 PMID: 3493353
  42. INDUCTION OF PARTIAL SPECIFIC HETEROTYPIC IMMUNITY IN MICE BY A SINGLE INFECTION WITH INFLUENZA A VIRUS.
    J Bacteriol. 1965 Jan;89:170-4 PMID: 14255658
Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.165.6.1508
Published
1987-06-01
Pages
1508-23
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2188365
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.165.6.1508 PMC Full Text View journal details
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